Distinct functions of integrin alpha and beta subunit cytoplasmic domains in cell spreading and formation of focal adhesions
Distinct functions of integrin alpha and beta subunit cytoplasmic domains in cell spreading and formation of focal adhesions
复制标题
整合素α和β亚基胞质结构域在细胞扩散和粘着斑形成中的独特功能
作者：
J. Ylänne;Yi;T. O’Toole;J. Loftus;Y. Takada;M. Ginsberg
Integrin-mediated cell adhesion often results in cell spreading and the formation of focal adhesions. We exploited the capacity of recombinant human alpha IIb beta 3 integrin to endow heterologous cells with the ability to adhere and spread on fibrinogen to study the role of integrin cytoplasmic domains in initiation of cell spreading and focal adhesions. The same constructs were also used to analyze the role of the cytoplasmic domains in maintenance of the fidelity of the integrin repertoire at focal adhesions. Truncation mutants of the cytoplasmic domain of alpha IIb did not interfere with the ability of alpha IIb beta 3 to initiate cell spreading and form focal adhesions. Nevertheless, deletion of the alpha IIb cytoplasmic domain allowed indiscriminate recruitment of alpha IIb beta 3 to focal adhesions formed by other integrins. Truncation of the beta 3 subunit cytoplasmic domain abolished cell spreading mediated by alpha IIb beta 3 and also abrogated recruitment of alpha IIb beta 3 to focal adhesions. This truncation also dramatically impaired the ability of alpha IIb beta 3 to mediate the contraction of fibrin gels. In contrast, the beta 3 subunit cytoplasmic truncation did not reduce the fibrinogen binding affinity of alpha IIb beta 3. Thus, the integrin beta 3 subunit cytoplasmic domain is necessary and sufficient for initiation of cell spreading and focal adhesion formation. Further, the beta 3 cytoplasmic domain is required for the transmission of intracellular contractile forces to fibrin gels. The alpha subunit cytoplasmic domain maintains the fidelity of recruitment of the integrins to focal adhesions and thus regulates their repertoire of integrins.
登录
查看更多内容
影响因子：
4
作者：
Karl R. Fath;C.-J. S. Edgell;Keith Burridge
通讯作者：
Karl R. Fath;C.-J. S. Edgell;Keith Burridge
影响因子：
56.9
作者：
HIBBS, ML;XU, H;SPRINGER, TA
通讯作者：
SPRINGER, TA
DOI：
10.1016/s0021-9258(18)42050-9
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Weisel;C. Nagaswami;G. Vilaire;J. Bennett
通讯作者：
J. Weisel;C. Nagaswami;G. Vilaire;J. Bennett
通过定点诱变绘制整合素 β1 胞质结构域的功能决定簇。
DOI：
10.1091/mbc.1.8.597
发表时间：
1990
期刊：
Cell regulation
影响因子：
--
作者：
Marcantonio,EE;Guan,JL;Trevithick,JE;Hynes,RO
通讯作者：
Hynes,RO
血小板 GPIIb-IIIa 的有效表面表达需要这两个亚基。
影响因子：
20.3
作者：
O'Toole,TE;Loftus,JC;Plow,EF;Glass,AA;Harper,JR;Ginsberg,MH
通讯作者：
Ginsberg,MH