An intrinsic oscillation in interneurons of the rat lateral geniculate nucleus.
An intrinsic oscillation in interneurons of the rat lateral geniculate nucleus.
复制标题
大鼠外侧膝状核中间神经元的内在振荡。
DOI：
10.1152/jn.1999.81.2.702
复制
发表时间：
1999
影响因子：
2.5
通讯作者：
D. Uhlrich
中科院分区：
文献类型：
--
作者：
J. J. Zhu;William W. Lytton;Jin;D. Uhlrich
采用体外全细胞膜片记录技术，观察了大鼠膝状体背外侧核(LGN)69个中间神经元的电压依赖性放电模式。当保持在超极化的膜电位时，所有中间神经元都以动作电位的爆发作出反应。在48个中间神经元中，较大的电流脉冲产生爆发性振荡。当相对去极化时，一些中间神经元对去极化电流脉冲产生一系列紧张性动作电位。然而，大多数中间神经元只产生振荡，与极化水平无关。对于兴奋性和抑制性突触传递的阻断剂组合，包括30微米6，7-二硝基-2，3-二酮，100微米(+/-)-2-氨基-5-膦戊酸，20微米荷包牡丹碱和2 mM舒氯芬，这种振荡不敏感，表明这是一种内源性事件。中间神经元的振荡频率取决于注入电流的强度。电流强度越大，振荡频率越高。对于大多数细胞来说，振荡的最大频率是5-15赫兹，由于很难准确定义从振荡行为到规则放电行为的转变，因此造成了一些模糊。相反，超极化和去极化脉冲对神经元间振荡的影响较小。除了由去极化电流注入引起的振荡外，当中间神经元保持在去极化的膜电位时，电刺激视束也可以引起这种振荡。这表明，中间神经元可能通过突触输入被招募到丘脑的振荡中。这些结果表明，中间神经元可能在丘脑振荡中发挥比之前认为的更大的作用。
By using the whole cell patch recording technique in vitro, we examined the voltage-dependent firing patterns of 69 interneurons in the rat dorsal lateral geniculate nucleus (LGN). When held at a hyperpolarized membrane potential, all interneurons responded with a burst of action potentials. In 48 interneurons, larger current pulses produced a bursting oscillation. When relatively depolarized, some interneurons produced a tonic train of action potentials in response to a depolarizing current pulse. However, most interneurons produced only oscillations, regardless of polarization level. The oscillation was insensitive to the bath application of a combination of blockers to excitatory and inhibitory synaptic transmission, including 30 microM 6,7-dinitroquinoxaline-2,3-dione, 100 microM (+/-)-2-amino-5-phosphonopentanoic acid, 20 microM bicuculline, and 2 mM saclofen, suggesting an intrinsic event. The frequency of the oscillation in interneurons was dependent on the intensity of the injection current. Increasing current intensity increased the oscillation frequency. The maximal frequency of the oscillation was 5-15 Hz for most cells, with some ambiguity caused by the difficulty of precisely defining a transition from oscillatory to regular firing behavior. In contrast, the interneuron oscillation was little affected by preceding depolarizing and hyperpolarizing pulses. In addition to being elicited by depolarizing current injections, the oscillation could also be initiated by electrical stimulation of the optic tract when the interneurons were held at a depolarized membrane potential. This suggests that interneurons may be recruited into thalamic oscillations by synaptic inputs. These results indicate that interneurons may play a larger role in thalamic oscillations than was previously thought.
影响因子：
2.5
作者：
MCCORMICK, DA;HUGUENARD, JR
通讯作者：
HUGUENARD, JR
影响因子：
2.5
作者：
R. Traub;R. Wong;R. Miles;H. Michelson
通讯作者：
R. Traub;R. Wong;R. Miles;H. Michelson