Disposition of cannabichromene, cannabidiol, and Δ⁹-tetrahydrocannabinol and its metabolites in mouse brain following marijuana inhalation determined by high-performance liquid chromatography-tandem mass spectrometry.
Disposition of cannabichromene, cannabidiol, and Δ⁹-tetrahydrocannabinol and its metabolites in mouse brain following marijuana inhalation determined by high-performance liquid chromatography-tandem mass spectrometry.
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通过高效液相色谱-串联质谱法测定吸入大麻后小鼠脑中大麻环烯、大麻二酚和α-四氢大麻酚及其代谢物的处置。
DOI：
10.1093/jat/34.8.516
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发表时间：
2010
影响因子：
2.5
通讯作者：
Poklis,Alphonse
中科院分区：
文献类型：
--
作者：
Poklis,JustinL;Thompson,CandaceC;Long,KellyA;Lichtman,AronH;Poklis,Alphonse
建立了一种液相色谱-串联质谱（LC-MS-MS）方法，用于分析小鼠脑组织中的大麻大麻素，该方法使用Applied Biosystems 3200 Q捕集器，该捕集器具有连接到Shimadzu SCL HPLC系统的TurbolonSpray的turbo V源。该方法包括大麻色烯（CBC）、大麻二酚（CBD）、Δ9-四氢大麻酚（THC）、11-羟基四氢大麻酚（11-OH-THC）和11-去甲-Δ9-四氢大麻酚-9-羧酸（THC-COOH）。这些化合物通过使用冷乙腈的液-液萃取分离。通过多反应监测（MRM）监测以下过渡离子：m/z315>193，315>259（对于THC/CBD/CBC）;m/z331>193，331>105（对于11-OH-THC）;m/z345>299，345>193（对于THC-COOH）;m/z318>196（对于THC-d3）;m/z334>196（对于11-OH-THC-d3），和m/z348>302（对于THC-COOH-d3）。THC、1-OH-THC和THC-COOH的线性范围为1-200 ng/g; CBC和CBD的线性范围为0.5-20 ng/g。所有分析物的运行内和运行间精密度产生的变异系数<20%。四只C57 BL 6小鼠在仅鼻暴露于含有0.44 mg CBC、0.93 mg CBD和8.81 mg THC的200 mg大麻烟雾后20分钟被处死。平均脑浓度为3.9 ± 1.5 ng/g CBC、21 ± 3.9 ng/g CBD、364 ± 74 ng/g THC和28 ± 5.9 ng/g 11-OH-THC。未检测到THC-COOH。相对平均脑大麻素浓度与吸入大麻中大麻素的量相关。
A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the analysis of marijuana cannabinoids in mouse brain tissue using an Applied Biosystems 3200 Q trap with a turbo V source for TurbolonSpray attached to a Shimadzu SCL HPLC system. The method included cannabichromene (CBC), cannabidiol (CBD), Δ9-tetrahydrocannabinol (THC), 11-hydroxytetrahydrocannabinol (11-OH-THC), and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH). These compounds were isolated by liquid-liquid extraction using cold acetonitrile. The following transition ions were monitored by multiple reaction monitoring (MRM):m/z315>193, 315>259 for THC/CBD/CBC;m/z331>193, 331>105 for 11-OH-THC;m/z345>299, 345>193 for THC-COOH;m/z318>196 for THC-d3;m/z334>196 for 11-OH-THC-d3, andm/z348>302 for THC-COOH-d3. Linearity for THC, 1-OH-THC, and THC-COOH was 1–200 ng/g; for CBC and CBD, it was 0.5–20 ng/g. Within-run and between-run precisions for all the analytes yielded coefficients of variation of < 20%. Four C57BL6 mice were sacrificed 20 min after nose-only exposure to the smoke of 200 mg of marijuana containing 0.44 mg CBC, 0.93 mg CBD, and 8.81 mg THC. The mean brain concentrations were 3.9 ± 1.5 ng/g CBC, 21 ± 3.9 ng/g CBD, 364 ± 74 ng/g THC, and 28 ± 5.9 ng/g 11-OH-THC. THC-COOH was not detected. The relative mean brain cannabinoid concentrations correlated to the amounts of the cannabinoids in the inhaled marijuana.
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影响因子：
4.2
作者：
Lichtman, AH;Poklis, JL;Martin, BR
通讯作者：
Martin, BR
影响因子：
2
作者：
Hoffman, Alexander F.;Oz, Murat;Lupica, Carl R.
通讯作者：
Lupica, Carl R.
使用毛细管柱气相色谱负离子化学电离质谱法定量测量生理标本中的 δ9-四氢大麻酚和两种主要代谢物。
DOI：
--
发表时间：
1983
期刊：
Biomedical mass spectrometry
影响因子：
--
作者：
R. Foltz;K. McGinnis;D. Chinn
通讯作者：
D. Chinn
影响因子：
2.2
作者：
Brunet, Bertrand;Doucet, Carole;Mura, Patrick
通讯作者：
Mura, Patrick
影响因子：
5.1
作者：
Radwan MM;Elsohly MA;Slade D;Ahmed SA;Khan IA;Ross SA
通讯作者：
Ross SA