MicroRNA-375 targets Hippo-signaling effector YAP in liver cancer and inhibits tumor properties
MicroRNA-375 targets Hippo-signaling effector YAP in liver cancer and inhibits tumor properties
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DOI：
10.1016/j.bbrc.2010.03.036
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发表时间：
2010-04-09
影响因子：
3.1
通讯作者：
Luk, John M.
中科院分区：
文献类型：
--
作者：
Liu, Angela M.;Poon, Ronnie T. P.;Luk, John M.
Hepatocellular carcinoma (HCC) is a malignant form of liver cancer that ranks the second leading cause of cancer-related deaths in China and many Asia regions The dismal outcome reflects the need for a better understanding of the transcriptional control of oncogenic signaling pathway. Our recent findings have identified yes-associated protein (YAP) is a potent oncogenic driver and independent prognostic risk factor of HCC The present study aims to elucidate the transcriptional regulation of YAP targeted by microRNA (miRNA) miR-375 is a putative target and was found significantly down-regulated in the tumor versus adjacent non-tumor tissues of HCC patients (n = 48) As determined by luciferase reporter assay, we found ectopic expression of miR-375 could diminish the transcriptional activity of YAP Furthermore, immunoblotting revealed miR-375 suppressed endogenous YAP protein level Functional assays showed that miR-375 was able to inhibit proliferation and invasion of HCC cells Conclusion miR-375 is an important regulator of YAP oncogene. implicating a potential therapeutic role in HCC treatment (C) 2010 Elsevier Inc All rights reserved.
