Effects of bilobalide on amino acid release and electrophysiology of cortical slices
Effects of bilobalide on amino acid release and electrophysiology of cortical slices
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DOI：
10.1007/s007260200021
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发表时间：
2002-01-01
期刊：
影响因子：
3.5
通讯作者：
Davies, JA
中科院分区：
文献类型：
--
作者：
Jones, FA;Chatterjee, SS;Davies, JA
本研究探讨银杏内酯，银杏叶的一种成分，对钾和藜芦碱诱导的小鼠皮层脑片谷氨酸和天冬氨酸释放的影响。我们还研究了其对自发和N-甲基-D-天冬氨酸（NMDA）诱导的去极化引起的无镁人工脑脊液（aCSF），以及其对NO-711（γ-氨基丁酸（GABA）摄取抑制剂）诱导的去极化的影响。白果内酯，100 μ M显着降低谷氨酸和天冬氨酸释放引起的钾或藜芦碱。白果内酯（5- 100 μ M）也显著降低NO-711诱导的去极化频率，然而，它在5- 200 μ M时对自发或NMDA诱导的去极化没有影响。这些结果表明，白果内酯的神经活性可能是通过减少兴奋性氨基酸神经递质的释放来介导的。
This study investigated the effects of bilobalide, a constituent of Ginkgo biloba, on potassium and veratridine-induced release of glutamate and aspartate from mouse cortical slices. We also studied its effects on spontaneous and N-methyl-D-aspartate (NMDA)-induced depolarizations elicited in magnesium-free artificial cerebrospinal fluid (aCSF) as well as its effect on NO-711 (a gamma-aminobutyric acid (GABA) uptake inhibitor) -induced depolarizations. Bilobalide, 100muM significantly reduced both glutamate and aspartate release elicited by potassium or veratridine. Bilobalide (5-100muM) also significantly reduced the frequency of NO-711 induced depolarizations, however, it had no effect on spontaneous or on NMDA-induced depolarizations at 5-200muM. These results suggest that the neuroactive properties of bilobalide may be mediated by a reduction in excitatory amino acid neurotransmitter release.