Autoinducer-2 bioassay is a qualitative, not quantitative method influenced by glucose
Autoinducer-2 bioassay is a qualitative, not quantitative method influenced by glucose
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DOI：
10.1016/j.mimet.2006.02.001
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发表时间：
2006-09-01
影响因子：
2.2
通讯作者：
Chikindas, Michael L.
中科院分区：
文献类型：
--
作者：
Turovskiy, Yevgeniy;Chikindas, Michael L.
自诱导物-2（AI-2）是一种细胞间信号传导分子，被认为是多种原核生物用于群体感应过程。这种分子通常使用所谓的自诱导物生物测定来检测，该生物测定依赖于哈氏弧菌报告菌株响应AI-2产生光的能力。然而，如先前所报道的，样品中葡萄糖的存在可以抑制报告菌株的生物发光，这一事实常常被研究者忽视。我们的数据表明，葡萄糖浓度低于抑制生物发光所需的浓度可能会导致AI-2读数不正确，并产生误导性（假阳性）结果。我们的研究结果还表明，即使考虑到这种生物测定的所有限制，该方法的大标准偏差仅允许对所得结果进行定性而非定量解释。(c)2006 Elsevier B.V保留所有权利。
Autoinducer-2 (AI-2) is a cell-to-cell signaling molecule which is thought to be utilized for quorum sensing processes by a variety of prokaryotic species. This molecule is usually detected using a so-called autoinducer bioassay, which relies on the ability of a Vibrio harveyi reporter strain to produce light in response to AI-2. However, as previously reported, the presence of glucose in the sample can inhibit the bioluminescence of the reporter strain, a fact that is often ignored by investigators. Our data suggest that the presence of glucose in concentrations below that required for the inhibition of bioluminescence may lead to incorrect AI-2 readings and produce misleading (false-positive) results. Our findings also suggest that even if all the limitations of this bioassay are considered, the large standard deviation of the method allows only for a qualitative and not quantitative interpretation of the obtained results. (c) 2006 Elsevier B.V All rights reserved.