The future of human DNA vaccines.
The future of human DNA vaccines.
复制标题
人DNA疫苗的未来。
DOI：
10.1016/j.jbiotec.2012.08.012
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发表时间：
2012-12-31
影响因子：
4.1
通讯作者：
Petrovsky, Nikolai
中科院分区：
文献类型：
--
作者：
Li, Lei;Saade, Fadi;Petrovsky, Nikolai
DNA疫苗自发明以来在过去20年中已经有了很大的发展，但尚未成为传统的基于蛋白质或碳水化合物的人类疫苗的竞争性替代品。虽然安全性问题是最初的障碍，但与蛋白质疫苗相比，DNA疫苗的致命弱点仍然是其免疫原性差。已经开发了各种各样的策略来优化DNA疫苗的免疫原性，包括密码子优化、遗传佐剂、电穿孔和复杂的初免-加强方案，这些方法中的每一种都有其优点和局限性。虽然这些方法中的每一种都有助于DNA疫苗功效的逐步改善，但如果人类DNA疫苗要在商业上取得成功，仍然需要更多。本文认为，人类DNA疫苗的最终突破将来自于最新前沿技术的应用，包括“表观遗传学”和“组学”方法，以及传统的提高免疫原性的技术，如佐剂和电穿孔，从而克服目前人类DNA疫苗的局限性。
DNA vaccines have evolved greatly over the last 20 years since their invention, but have yet to become a competitive alternative to conventional protein or carbohydrate based human vaccines. Whilst safety concerns were an initial barrier, the Achilles heel of DNA vaccines remains their poor immunogenicity when compared to protein vaccines. A wide variety of strategies have been developed to optimize DNA vaccine immunogenicity, including codon optimization, genetic adjuvants, electroporation and sophisticated prime-boost regimens, with each of these methods having its advantages and limitations. Whilst each of these methods has contributed to incremental improvements in DNA vaccine efficacy, more is still needed if human DNA vaccines are to succeed commercially. This review foresees a final breakthrough in human DNA vaccines will come from application of the latest cutting-edge technologies, including “epigenetics” and “omics” approaches, alongside traditional techniques to improve immunogenicity such as adjuvants and electroporation, thereby overcoming the current limitations of DNA vaccines in humans
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