Elevated expression of miR-21 and miR-155 in peripheral blood mononuclear cells as potential biomarkers for lupus nephritis
Elevated expression of miR-21 and miR-155 in peripheral blood mononuclear cells as potential biomarkers for lupus nephritis
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DOI：
10.1111/1756-185x.13410
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发表时间：
2019-03-01
影响因子：
2.5
通讯作者：
Shekarabi, Mehdi
中科院分区：
文献类型：
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作者：
Khoshmirsafa, Majid;Kianmehr, Nahid;Shekarabi, Mehdi
目的狼疮性肾炎（LN）是系统性红斑狼疮（SLE）的严重并发症。在自身免疫性疾病中使用microRNAs（miRNAs）作为诊断和预后生物标志物具有极大的兴趣。材料与方法本研究采用定量聚合酶链反应（PCR）检测了55例女性SLE患者（无肾炎、活动性肾炎或活动性肾炎）和30例健康对照（HC）外周血单个核细胞（PBMC）中miR-16、miR-21、miR-141、miR-146 a和miR-155的表达水平。结果活动性肾炎组miR-21和miR-155水平显著高于无活动性肾炎组、非活动性肾炎组和HC组。此外，受试者操作特征和logistic回归分析显示，miR-21和miR-155是LN的重要危险因素。结论miR-21和miR-155在PBMCs中的高表达可能参与了LN的病理生理过程，这些miRNA可作为LN的生物标志物。
Aim Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE). There is a great interest in using microRNAs (miRNAs) as diagnostic and prognostic biomarkers in autoimmune diseases. Materials and methods This study evaluated miR-16, miR-21, miR-141, miR-146a, and miR-155 expression levels in peripheral blood mononuclear cells (PBMCs) of 55 female SLE patients with absent, inactive, or active nephritis, and 30 healthy controls (HCs) using quantitative polymerase chain reaction. Results MiR-21 and miR-155 levels were significantly greater in the active nephritis group than in the absent, inactive or HC groups. Moreover, receiver operating characteristic and logistic regression analyses revealed miR-21 and miR-155 were significant risk factors for LN. Conclusion Overexpression of miR-21 and miR-155 in PBMCs may participate in LN pathophysiology and these miRNAs could be used as biomarkers for the condition.