Microparticle-mediated RNA immunization against bovine viral diarrhea virus
Microparticle-mediated RNA immunization against bovine viral diarrhea virus
复制标题
DOI：
10.1016/s0264-410x(00)00438-2
复制
发表时间：
2001-02-28
期刊：
影响因子：
5.5
通讯作者：
Donis, RO
中科院分区：
文献类型：
--
作者：
Vassilev, VB;Gil, LHVG;Donis, RO
牛病毒性腹泻病毒（BVDV）NADL株全长感染性克隆的感染性转录物用于接种牛和绵羊以对抗BVDV。通过用Hellos基因枪微粒轰击递送的体外合成RNA启动BVDV复制，并因此诱导针对I型BVDV（血清中和滴度，SNT > 2（12））和II型BVDV（SNT > 2（7））的体液免疫。通过组织培养单层的微粒轰击来评估RNA-载体复合物的质量和长期稳定性。发现RNA检测盒在储存后可稳定至少8个月。这是第一次成功的RNA疫苗接种大型反刍动物的报告。(C)2001爱思唯尔科技有限公司版权所有。
Infectious transcripts from the full-length infectious clone of the NADL strain of bovine viral diarrhea virus (BVDV) were used to vaccinate cattle and sheep against BVDV. In vitro synthesized RNA delivered by microparticle bombardment with a Hellos Gene Gun initiated replication of BVDV and consequently induced humoral immunity against type I BVDV (serum neutralization titers, SNT > 2(12)) and type II BVDV (SNT > 2(7)). The quality and long-term stability of the RNA-carrier complexes was assessed by microparticle bombardment of tissue culture monolayers. The RNA cartridges were found to be stable for at least 8 months upon storage. This is the first report on successful RNA vaccination of large ruminants. (C) 2001 Elsevier Science Ltd. All rights reserved.