Marine glycosyl hydrolases in the hydrolysis and synthesis of oligosaccharides
Marine glycosyl hydrolases in the hydrolysis and synthesis of oligosaccharides
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DOI：
10.1002/biot.200500036
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发表时间：
2006-05-01
影响因子：
4.7
通讯作者：
Trincone, Antonio
中科院分区：
文献类型：
--
作者：
Giordano, Assurita;Andreotti, Giuseppina;Trincone, Antonio
海洋生态系统可以被认为是一个相当未开发的生物材料来源（例如具有治疗活性的天然物质），也可以是一个令人惊讶的酶来源，这些酶具有新的和有趣的催化活性，可用于生物催化。来自海洋环境的糖基水解酶的使用可以追溯到20世纪60年代末，主要集中在开发用于糖链分析的灵敏可靠的水解方法。因此，尚未研究特定酶活性的所有益处，特别是关于这些酶用于合成糖苷键的转糖基化潜力。在这篇综述中，海洋来源的潜力将被证明报告在文献中发现的几个例子的合成和水解的生物相关的寡糖催化的糖基水解酶的海洋来源。特别强调的是糖苷键的合成，这是很容易通过使用糖基水解酶。在这篇评论中考虑的进一步方面是这些生物催化剂的应用，植物废物处理回收有用的材料，结构鉴定和制备的目标材料从新的纯化多糖，食品相关化合物的合成或修饰和糖生物学相关的研究。
The marine ecosystem can be considered a rather unexplored source of biological material (e.g. natural substances with therapeutic activity) and can also be a surprising source of enzymes carrying new and interesting catalytic activities to be applied in biocatalysis. The use of glycosyl hydrolases from marine environments dates back to the end of the 1960s and was mainly focused on the development of sensitive and reliable hydrolytic methods for the analysis of sugar chains. As a result not all the benefits of a particular enzymatic activity have been investigated, especially regarding the transglycosylation potential of these enzymes for the synthesis of glycosidic bonds. In this review, the potential of marine sources will be demonstrated reporting on the few examples found in literature for the synthesis and hydrolysis of biologically relevant oligosaccharides catalyzed by glycosyl hydrolases of marine origin. Particular emphasis is given to the synthesis of glycosidic bonds, which is easy by the use of glycosyl hydrolases. Further aspects considered in this review are applications of these biocatalysts for vegetal waste treatment in recovering useful materials, for structural identification and for preparation of target materials from new purified polysaccharides, for the synthesis or modification of food-related compounds and for glycobiology related studies.