In vitro nematicidal effects of medicinal plants from the Sierra de Huautla, Biosphere Reserve, Morelos, Mexico against Haemonchus contortus infective larvae
In vitro nematicidal effects of medicinal plants from the Sierra de Huautla, Biosphere Reserve, Morelos, Mexico against Haemonchus contortus infective larvae
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DOI：
10.1017/s0022149x07873627
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发表时间：
2008-03-01
影响因子：
1.6
通讯作者：
Hernandez-Velazquez, V.
中科院分区：
文献类型：
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作者：
Lopez-Aroche, U.;Salinas-Sanchez, D. O.;Hernandez-Velazquez, V.
对来自墨西哥莫雷洛斯胡特拉山脉生物圈保护区的20种植物提取物进行了体外抗缠绕血吸虫幼虫试验。所评价的植物种类有：油菜籽、大叶油菜、鹅掌菜、墨西哥西番莲、宽叶山毛蛙、棘皮林蛙和大叶荨麻。这些植物被分成不同的部分，用不同的溶剂(正己烷、丙酮、乙醇和甲醇)浸泡。用体外驱虫法测定其对脱鞘第三期螺旋体感染幼虫的驱虫活性。实验在24孔细胞培养板上进行，室温下，每次处理重复3次，浓度为20 mg·ml~(-1)。分别于照射后24、48、72、96h取10只5亩L等份标本，放在玻片上进行显微镜观察。评价标准是根据不同处理的平均活幼虫和/或死幼虫数量。活体幼虫和死亡幼虫的数量通过方差分析进行统计学分析(P>；0.01)。Tukey检验被用作确定哪个处理与其他处理不同的补充工具(P>；0.05)。雷公藤茎叶总萃取物的死亡率最高，24、48和72h幼虫死亡率分别为51%、81%和86%。另一方面，铜绿假单胞菌茎丙酮提取物在暴露24、48和72h后分别表现出18%、59%和66%的杀线虫活性。
Twenty extracts from plants from Sierra de Huautla Biosphere Reserve, Morelos, Mexico were evaluated against Haemonchus contortus infective larvae in an in vitro assay. The plant species evaluated were Bursera copallifera, B. grandifolia, Lippia graveolens, Passiflora mexicana, Prosopis laevigata, Randia echinocarpa and Urtica dioica. The plants were separated into their parts and macerated with different solvents (n-hexane, acetone, ethanol and methanol). An in vitro assay was used to evaluate the anthelmintic activity against unsheathed third stage H. contortus infective larvae. The experiment was carried out in 24-well cell culture plates at room temperature with three replicates per treatment and using a concentration of 20 mg ml(-1). Ten 5 mu l aliquots were taken from the corresponding wells and deposited on a slide for microscopical observation at 24, 48, 72 and 96 h post-exposure. The evaluation criteria were based on the average numbers of live and/or dead larvae in the different treatments. Alive and dead larval numbers were statistically analysed through the ANOVA test (P > 0.01). The Tukey test was used as a complementary tool to determine which treatment was different from the other treatments (P > 0.05). The highest mortality was observed with R laevigata hexanic extract from stem and leaves combined, which produced 51%, 81% and 86% larval mortality at 24, 48 and 72h post-exposure, respectively. On the other hand, B. copallifera stem acetonic extract exhibited 18%, 59% and 66% nematicidal activity after 24, 48 and 72h of exposure, respectively.