Experimental approaches to study vascularization in tissue engineering and biomaterial applications
Experimental approaches to study vascularization in tissue engineering and biomaterial applications
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DOI：
10.1023/a:1024903514335
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发表时间：
2003-08-01
影响因子：
3.7
通讯作者：
Kamp, G
中科院分区：
文献类型：
--
作者：
Kirkpatrick, CJ;Unger, RE;Kamp, G
组织工程和生物材料应用的成功不仅取决于生物材料上器官或组织特异性细胞的生长和功能，而且在大多数情况下完全取决于植入后成功的血管形成。血管化的过程包括血管生成；新血管的形成扩散到植入材料中，并为现有细胞提供生存所需的营养物质。我们已经建立了使用人微血管内皮细胞的体外方法来评估新型生物材料对内皮细胞的附着、细胞毒性、生长、血管生成以及对基因调控的影响。这些体外研究可以用来快速评估一种新的生物材料的潜在成功，并用于开发促进生理血管形成反应的基质支架。(C)2003年克鲁沃学术出版社
The success of tissue engineering and biomaterial applications is not only dependent on the growth and functioning of the organ- or tissue-specific cells on the biomaterial but is entirely dependent in most cases on a successful vascularization after implantation. The process of vascularization involves angiogenesis; the formation of new blood vessels which spread into the implant material and supply the existing cells with the nutrients to survive. We have established in vitro methods using human microvascular endothelial cells to evaluate novel biomaterials for endothelial cell attachment, cytotoxicity, growth, angiogenesis and the effects on gene regulation. These in vitro studies can be used to rapidly evaluate the potential success of a new biomaterial and for the development of matrix scaffolds which will promote a physiological vascularization response. (C) 2003 Kluwer Academic Publisher