Ethidium Monoazide-Loop Mediated Isothermal Amplification for Rapid Detection of Vibrio parahaemolyticus in Viable but non-culturable State
Ethidium Monoazide-Loop Mediated Isothermal Amplification for Rapid Detection of Vibrio parahaemolyticus in Viable but non-culturable State
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单叠氮环介导的等温扩增可快速检测活但不可培养状态的副溶血弧菌
DOI：
10.1016/j.egypro.2012.02.323
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
王丽
中科院分区：
文献类型：
--
作者：
王丽
Loop-mediated isothermal amplification (LAMP) combined with the ethidium monoazide (EMA) treatment was applied for differentiation from VBNC state and dead state of V. parahaemolyticus. The tlh gene can be amplified remarkably within 1 h when the amount of DNA was 9fg in EMA-LAMP assay. EMA has the ability to penetrate selectively through the damaged cytoplasmic membrane of dead cells and to intercalate into DNA, so the gene of the dead cells could not be amplified. Amplification of DNA from dead bacterial was successfully inhibited by EMA, whereas the DNA derived from V. parahaemolyticus in VBNC state was readily amplified.
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影响因子：
2.1
作者：
Wang, Li;Li, Lin;Shi, Lei
通讯作者：
Shi, Lei
DOI：
10.1016/j.diagmicrobio.2009.09.004
发表时间：
2010-02-01
影响因子：
2.9
作者：
Okada, Kazuhisa;Chantaroj, Siriporn;Sawanpanyalert, Pathom
通讯作者：
Sawanpanyalert, Pathom
DOI：
--
发表时间：
2000
期刊：
Microbios
影响因子：
--
作者：
R. Levin;A. Abolmaaty;C. Vu;J. Oliver
通讯作者：
R. Levin;A. Abolmaaty;C. Vu;J. Oliver
影响因子：
2.4
作者：
Besnard, V;Federighi, M;Cappelier, JM
通讯作者：
Cappelier, JM
影响因子：
2.2
作者：
Lee, Jung-Lim;Levin, Robert E.
通讯作者：
Levin, Robert E.