Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging
Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging
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DOI：
10.1148/radiol.2293021215
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发表时间：
2003-12-01
期刊：
影响因子：
19.7
通讯作者：
Frank, JA
中科院分区：
文献类型：
--
作者：
Arbab, AS;Bashaw, LA;Frank, JA
目的：评价氧化亚铁-聚L-赖氨酸(PLL)复合体用于磁性细胞标记对哺乳动物细胞长期存活率、功能、代谢和铁利用的影响。材料和方法：将PLL与亚铁氧化物孵育60min，通过静电作用不完全包覆超顺磁性氧化铁(SPIO)。将细胞与亚铁氧化物-PLL复合体共同孵育过夜，评估铁摄取、细胞存活率、细胞凋亡指数和活性氧的形成。还对细胞中可检测到的铁纳米颗粒的消失或寿命进行了评估。在不同的时间点也评估了介质中的铁浓度。结果：亚铁氧化物-PLL复合体磁性标记人骨髓间充质干细胞和HeLa细胞时，胞质内纳米颗粒被普鲁士蓝染色。与非标记细胞的这些特征相比，标记细胞的长期存活率、生长速度和凋亡指数不受SPIO包涵体掺入的影响。在未分裂的人骨髓间充质干细胞中，7周后可以检测到包涵体铁纳米颗粒；然而，在快速分裂的细胞中，细胞内的铁已经消失了5到8个分裂。在人骨髓间充质干细胞和HeLa细胞系中，观察到活性氧物种的产生没有显著的瞬时增加。标记的人骨髓间充质干细胞不能分化为其他谱系。人骨髓间充质干细胞和HeLa细胞培养液中的铁浓度在培养7d时均显著升高。结论：铁氧化物-PLL复合体磁性细胞标记对肿瘤细胞和干细胞均无短期或长期毒性作用。-RSNA，2003年。
PURPOSE: To evaluate the effect of using the ferumoxides-POlY-L-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells.MATERIALS AND METHODS: PLIL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean +/- 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences.RESULTS: Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the enclosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, enclosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7.CONCLUSION: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells. - RSNA, 2003.