Glucose and infarct size
Glucose and infarct size
复制标题
DOI：
10.1212/wnl.41.12.2013
复制
发表时间：
1991-12
期刊：
影响因子：
9.9
通讯作者：
R. Swanson;F. Sharp
中科院分区：
文献类型：
--
作者：
R. Swanson;F. Sharp
致编辑：叶博士及其同事1描述了短暂性局灶性脑缺血后血浆ghxoee水平对梗死体积的影响。正如Dr. Yip等人的全面综述，mend研究者进行了s B k d a x B，但结论不一致（例如，参见Ginsberget dZ De Courten-Myers等人13）。我们希望提请注意一个方法上的问题，这可能是造成这些差异的部分原因。Yip博士和他的同事通过用氯化三苯基四氮唑（TTC）染色脑切片并测量未染色（梗死）组织的面积来确定梗死面积。虽然这种方法被广泛使用，但由于梗死组织的水肿肿胀，它必然会高估梗死面积。44肿胀程度是一个不受控制的变量，会随着固定方法、梗死年龄和其他因素而变化。水肿的量，以及表观梗死面积，也可能随实验变量如血浆渗透压，特别是脑乳酸含量而变化。这些影响的程度是否会明显改变这项或其他研究的结果尚不清楚，但这是可能的。5已经描述了一种消除这种潜在问题的简单方法。6实质上，该方法包括从每个脑切片的梗死侧面积中减去非梗死侧面积。差值代表水肿引起的面积增加，该值可用于校正表观梗死面积。采用这种方法可以减少此类实验中报告的结果的变异性。
To the Editor: Dr. Yip and colleagues1 describe the effect of plasma ghxoee levels on infard size following transient focal cerebral ischemia. As thoroughly reviewed by Dr. Yip et al, mend inveatigators have performed s b k d a x b but with d8eringconclusbns (eg, see Ginsberget dZ De Courten-Myers et a13). We wish to draw attention to a methodologic concern which may be in part responsible for these differences. Dr. Yip and colleagues determined infarct sizes by staining brain sections with triphenyltetrazolium chloride (TTC) and measuring the areas of unstained (infarct&) tissue. Although this approach is widely used, it necessarily overestimates infarct size due to edematous swelling of the infarcted tissue.44 The degree of swelling represents an uncontrolled variable that will vary with the fixation method, age of infarct, and other factors. The amount of edema, and thus the apparent infarct size, may also vary with experimental variables such as plasma osmolality and in particular with brain lactate content.? Whether the magnitude of these effects could appreciably alter the results of this or other studies is unknown, but it is possible.‘-5 A straightforward method of eliminating this potential problem has been described.6 In essence, the method involves subtracting the area of the noninfarcted side from the area of the infarcted side of each brain section. The difference represents the increased area attributable to edema, and this value can be used to correct the apparent infarct size. Adoption of this methodology may lessen the variability of results reported in experiments of this type.