Study of temperature-growth interactions of entomopathogenic fungi with potential for control of Varroa destructor (Acari: Mesostigmata) using a nonlinear model of poikilotherm development
Study of temperature-growth interactions of entomopathogenic fungi with potential for control of Varroa destructor (Acari: Mesostigmata) using a nonlinear model of poikilotherm development
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DOI：
10.1046/j.1365-2672.2003.01871.x
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Chandler, D
中科院分区：
文献类型：
--
作者：
Davidson, G;Phelps, K;Chandler, D
目的：研究欧洲蜜蜂蜜蜂体外寄生虫Varroa destructor的热生物学特性。方法和结果：测定了41株昆虫病原真菌在20℃、30℃和35℃3种温度下的菌落扩展速率。所有菌株都在20℃和30℃下生长，但只有11个菌株在35℃下生长。然后根据在30-35℃(蜜蜂群体内的温度范围)可察觉的生长和/或对破坏弧菌的感染性筛选出22个菌株，并在10℃(12.5-35℃)下测量它们的菌落延伸率。然后将这些数据与Schoolfield等人进行了拟合。夏普和德米歇尔[J Theor Biol(1981)88：719-731]变温发展模型的重新表述[J Theor Biol(1977)：649-670].总体而言，该模型解释了数据方差的87.6-93.9%。11个菌株的生长温度在35℃以上。伸长率的最适温度为22.9~31.2℃。只有3个菌株的最适温度在30℃以上。最适温度范围为31.9~43.2℃，最适温度为最适温度的10%。结论：被检菌群对温度的要求与蜜蜂群体无巢区温度匹配良好，部分菌株也应能在蜂巢内发挥作用。本研究所采用的方法可用于在筛选感染性之前用于微生物控制的菌株的选择，并有助于预测在变温条件下破坏弧菌的真菌控制剂的活性。
Aims: To investigate the thermal biology of entomopathogenic fungi being examined as potential microbial control agents of Varroa destructor , an ectoparasite of the European honey bee Apis mellifera .Methods and Results: Colony extension rates were measured at three temperatures (20, 30 and 35degreesC) for 41 isolates of entomopathogenic fungi. All of the isolates grew at 20 and 30degreesC but only 11 isolates grew at 35degreesC. Twenty-two isolates were then selected on the basis of appreciable growth at 30-35degreesC (the temperature range found within honey bee colonies) and/or infectivity to V. destructor , and their colony extension rates were measured at 10 temperatures (12.5-35degreesC). This data were then fitted to Schoolfield et al . [J Theor Biol (1981)88:719-731] re-formulation of the Sharpe and DeMichele [J Theor Biol (1977)64:649-670] model of poikilotherm development. Overall, this model accounted for 87.6-93.9% of the data variance. Eleven isolates exhibited growth above 35degreesC. The optimum temperatures for extension rate ranged from 22.9 to 31.2degreesC. Only three isolates exhibited temperature optima above 30degreesC. The super-optimum temperatures (temperature above the optimum at which the colony extension rate was 10% of the maximum rate) ranged from 31.9 to 43.2degreesC.Conclusions: The thermal requirements of the isolates examined against V. destructor are well matched to the temperatures in the broodless areas of honey bee colonies, and a proportion of isolates, should also be able to function within drone brood areas.Significance and Impact of the Study: Potential exists for the control of V. destructor with entomopathogenic fungi in honey bee colonies. The methods employed in this study could be utilized in the selection of isolates for microbial control prior to screening for infectivity and could help in predicting the activity of a fungal control agent of V. destructor under fluctuating temperature conditions.