Tolerance of Sargassum thunbergii germlings to thermal, osmotic and desiccation stress
Tolerance of Sargassum thunbergii germlings to thermal, osmotic and desiccation stress
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马尾藻幼苗对热、渗透和干燥胁迫的耐受性
DOI：
10.1016/j.aquabot.2011.09.002
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发表时间：
2012-01-01
期刊：
影响因子：
1.8
通讯作者：
Yu, Yong Qiang
中科院分区：
文献类型：
--
作者：
Chu, Shao Hua;Zhang, Quan Sheng;Yu, Yong Qiang
由于极端的物理压力，在潮间带建造人工海藻床是一项挑战。为了给利用显微幼体的扩散构建藻体提供依据，采用三因素析因试验设计，研究了鼠尾藻（Sargassum thunberthums）可育体短期释放出的幼体对温度、盐度和干燥的耐受性。结果表明，温度、盐度和干燥度对幼鱼的生长和存活有显著影响。当在25 ℃和正常海水中完全浸泡培养时，幼苗表现出快速生长，相对生长率（RGR，% d（-1））超过16%。虽然生长受到中度条件下的幼苗显着抑制，RGRs超过13%。只有在35 ℃和9 h的干燥处理下，才观察到低于10%的幼苗RGR。与生长相比，生存受物理压力的影响较小。实验结束时，在适当条件下（25 ℃和30 ℃与完全浸泡相结合），幼苗显示出低于10%的低死亡率，在中等条件下低于60%。然而，在极端条件下（9小时干燥和35 ℃结合完全浸泡在盐度12），死亡率增加到90%以上。结果表明，S.结果表明，雷柏幼苗对物理胁迫具有较强的耐受性。除主效应外，温度、盐度和干化之间的双向和三向交互作用均显著。根据影响的大小，干燥是影响生长和存活的主要因素。根据研究结果，在自然栖息地建造人工水槽以减少干燥可能是一种有效的策略。利用幼苗进行的雷柏恢复。(C)2011 Elsevier B. V.保留所有权利。
The construction of artificial seaweed beds in the intertidal zone is a challenge due to extreme levels of physical stress. In order to provide a basis for the construction using the dispersal of microscopic juveniles, a three-way factorial experimental design was used to evaluate the tolerance of Sargassum thunbergii germlings shortly released from fertile thalli to temperature, salinity and desiccation in this study. Results revealed that temperature, salinity and desiccation significantly affected the growth and survival of germlings. Germlings showed rapid growth with relative growth rate (RGR, % day(-1)) over 16% when cultured at 25 degrees C and full immersion in normal seawater. Although growths of germlings subjected to moderate conditions were significantly inhibited, RGRs over 13% were obtained. The RGRs of germlings below 10% were observed only at 35 degrees C and 9 h desiccation treatments. In comparison to growth, survival was less affected by physical stress. Germlings showed low mortalities below 10% under appropriate conditions (25 degrees C and 30 degrees C combined with full immersion), and below 60% under moderate conditions, by the end of experiment. However, the mortality rates increased to over 90% under extreme conditions (9 h desiccation and 35 C combined with full immersion in salinity of 12). These results showed that S. thunbergii germlings had high tolerance to physical stresses. In addition to the main effects, both two-way and three-way interactions between temperature, salinity and desiccation were significant. Based on the magnitude of effect, desiccation was the predominant factor affecting both growth and survival. According to the results, construction of artificial tanks in natural habitat to minimize desiccation may be an effective strategy for S. thunbergii restoration using germlings. (C) 2011 Elsevier B.V. All rights reserved.