Genomic and functional analysis of the type VI secretion system in Acinetobacter.
Genomic and functional analysis of the type VI secretion system in Acinetobacter.
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DOI：
10.1371/journal.pone.0055142
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Feldman MF
中科院分区：
文献类型：
--
作者：
Weber BS;Miyata ST;Iwashkiw JA;Mortensen BL;Skaar EP;Pukatzki S;Feldman MF
不动杆菌属由一组不同的物种组成，其中几种由于在生物修复和农业目的中的潜在应用而引起了人们的兴趣。在这项工作中，我们表明，许多物种内的不动杆菌属具有遗传要求，组装一个功能VI型分泌系统（T6SS）。这种分泌系统在革兰氏阴性细菌中广泛存在，并且可以用于对其他细菌和真核细胞的毒性。该属中研究最多的物种是A.鲍曼不动杆菌是一种新出现的医院病原体，已成为全球医疗保健系统的重大威胁。A.鲍曼不动杆菌产生多药耐药性已严重减少了治疗选择，并且对大多数临床上有用的抗生素具有耐药性的菌株经常被分离出来。尽管A.对于鲍曼不动杆菌，人们对这种细菌用来引起感染的毒力因子知之甚少。我们确定T6SS在A.虽然标志蛋白Hcp的表达和分泌在菌株之间变化，并且依赖于TssM，T6SS功能所需的已知结构蛋白。与其他细菌不同，A.鲍曼不动杆菌ATCC 17978似乎不使用其T6 SS来杀死大肠杆菌或其他不动杆菌属物种。在包括小鼠在内的几种感染模型中，tssM的缺失不影响毒力，也不改变生物膜的形成。这些结果表明，T6SS履行一个重要的，但尚未确定的作用，在各种生活方式的不动杆菌属。
The genus Acinetobacter is comprised of a diverse group of species, several of which have raised interest due to potential applications in bioremediation and agricultural purposes. In this work, we show that many species within the genus Acinetobacter possess the genetic requirements to assemble a functional type VI secretion system (T6SS). This secretion system is widespread among Gram negative bacteria, and can be used for toxicity against other bacteria and eukaryotic cells. The most studied species within this genus is A. baumannii, an emerging nosocomial pathogen that has become a significant threat to healthcare systems worldwide. The ability of A. baumannii to develop multidrug resistance has severely reduced treatment options, and strains resistant to most clinically useful antibiotics are frequently being isolated. Despite the widespread dissemination of A. baumannii, little is known about the virulence factors this bacterium utilizes to cause infection. We determined that the T6SS is conserved and syntenic among A. baumannii strains, although expression and secretion of the hallmark protein Hcp varies between strains, and is dependent on TssM, a known structural protein required for T6SS function. Unlike other bacteria, A. baumannii ATCC 17978 does not appear to use its T6SS to kill Escherichia coli or other Acinetobacter species. Deletion of tssM does not affect virulence in several infection models, including mice, and did not alter biofilm formation. These results suggest that the T6SS fulfils an important but as-yet-unidentified role in the various lifestyles of the Acinetobacter spp.
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影响因子：
6.7
作者：
Iwashkiw JA;Seper A;Weber BS;Scott NE;Vinogradov E;Stratilo C;Reiz B;Cordwell SJ;Whittal R;Schild S;Feldman MF
通讯作者：
Feldman MF
影响因子：
6.4
作者：
Iwashkiw JA;Fentabil MA;Faridmoayer A;Mills DC;Peppler M;Czibener C;Ciocchini AE;Comerci DJ;Ugalde JE;Feldman MF
通讯作者：
Feldman MF
影响因子：
3.1
作者：
Aubert, Daniel F.;Flannagan, Ronald S.;Valvano, Miguel A.
通讯作者：
Valvano, Miguel A.
影响因子：
3.4
作者：
Choi, CH;Lee, EY;Lee, JC
通讯作者：
Lee, JC
影响因子：
3.1
作者：
Gaddy, Jennifer A.;Arivett, Brock A.;Actis, Luis A.
通讯作者：
Actis, Luis A.