A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli
A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli
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DOI：
10.1128/jb.178.10.2994-2997.1996
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发表时间：
1996-05
影响因子：
3.2
通讯作者：
K. Nakashima;K. Kanamaru;T. Mizuno;K. Horikoshi
中科院分区：
文献类型：
--
作者：
K. Nakashima;K. Kanamaru;T. Mizuno;K. Horikoshi
大肠杆菌含有主要的冷休克蛋白CspA（或CS7.4），其产生主要在低温下诱导。已知这种细菌具有五个额外的基因，每个基因编码与CspA高度相似的蛋白质（称为CspA家族）。在这里，我们确定了一个基因，编码CspA和CspB的冷休克诱导类似物。新克隆的cspG基因定位于E. coli的遗传图谱，除了其他cspA家族基因。其基因产物（70个氨基酸）分别与CspA（70个氨基酸）和CspB（71个氨基酸）具有73%和77%的同一性。cspG与lacZ的转录融合和北方（RNA）杂交分析表明cspG是一个低温响应基因。结果表明，cspG序列与cspA和cspB序列不仅在编码区高度相似，而且在其启动子周围的5 '上游非编码区也高度相似。
Escherichia coli contains a major cold shock protein, CspA (or CS7.4), whose production is predominantly induced at low temperatures. This bacterium is known to possess five additional genes, each encoding a protein highly similar to CspA (referred to as the CspA family). Here we identified a gene that encodes a cold-shock-inducible analog of CspA and CspB. This newly cloned cspG gene is located at 22 min on the E. coli genetic map, apart from the other cspA family genes. Its gene product (70 amino acids) is 73 and 77% identical to CspA (70 amino acids) and CspB (71 amino acids), respectively. Analyses of a cspG-lacZ transcriptional fusion and Northern (RNA) hybridization revealed that cspG is a low-temperature-responsive gene. Its low-temperature-inducible promoters were determined, and the results indicated that the cspG sequence is highly similar to both the cspA and cspB sequences not only in the coding regions but also in the 5'-upstream noncoding regions surrounding their own promoters.