TTX-resistant Ca2+ oscillation in cultured hypothalamus: similarity to the mammalian circadian pacemaker.
TTX-resistant Ca2+ oscillation in cultured hypothalamus: similarity to the mammalian circadian pacemaker.
复制标题
培养的下丘脑中 TTX 抗性 Ca2 振荡：与哺乳动物昼夜节律起搏器相似。
作者：
S. Yamazaki;S. T. Inouye;Y. Kuroda
分离培养的大鼠下丘脑细胞显示细胞内钙离子自发振荡，周期为5-8个S(超常节律)。在培养液中用重水代替H_2O延长了这种振荡的周期，而河豚毒素不能抑制重水敏感的钙振荡。由于这些特征类似于哺乳动物的昼夜节律，超常的钙节律可能参与了昼夜节律，或者具有相似的节律生成机制。缝隙连接抑制剂氟烷阻止了这种振荡。为了产生有节奏的振荡，缝隙连接可能是必要的。
Dissociated cultures of rat hypothalamic cells showed spontaneous oscillations in intracellular Ca2+ with periods of 5-8 s (ultradian rhythm). Replacement of H2O by deuterium oxide in the culture medium lengthened the period of this oscillation, while tetrodotoxin did not inhibit the deuterium oxide sensitive Ca2+ oscillation. Since these characteristics resemble those of the mammalian circadian pacemaker, the ultradian Ca2+ rhythms may be involved in the circadian rhythm or have a similar rhythm generation mechanism. The gap junction inhibitor, halothane, stopped this oscillation. Gap junctions may be necessary in order to generate rhythmic oscillations.