PRIMARY STRUCTURE OF HUMAN INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN PLACENTAL PROTEIN 12 AND TISSUE-SPECIFIC EXPRESSION OF ITS MESSENGER-RNA
PRIMARY STRUCTURE OF HUMAN INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN PLACENTAL PROTEIN 12 AND TISSUE-SPECIFIC EXPRESSION OF ITS MESSENGER-RNA
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DOI：
10.1016/0014-5793(88)80041-3
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发表时间：
1988-08-29
期刊：
影响因子：
3.5
通讯作者：
KONTULA, K
中科院分区：
文献类型：
--
作者：
JULKUNEN, M;KOISTINEN, R;KONTULA, K
低分子量胰岛素样生长因子结合蛋白（IGF-BP）和胎盘蛋白12（PP 12）是存在于人血清、羊水、分泌期子宫内膜和蜕膜中的相同蛋白质。IGF-BP/PP 12被认为是细胞生长的自分泌或旁分泌调节剂。从人蜕膜cDNA文库中分离出包含该蛋白的整个蛋白编码区的cDNA克隆。通过体外转录/翻译实验和通过推导的成熟肽的10个N-末端氨基酸与通过直接蛋白质测序获得的氨基酸的同一性来验证cDNA的真实性。氨基酸序列分析表明，前-IGF-BP/PP 12由259个氨基酸残基组成。推定的信号肽是25个残基长，和成熟的蛋白质，因此包含234个氨基酸，并具有25 293 Da的分子量。序列是非常丰富的半胱氨酸在N-末端之后，有集群的Pro，Glu，Ser和Thr残基的区域（所谓的PEST区域），存在于蛋白质中具有短的半衰期。该氨基酸序列还包括可用作细胞识别信号的Arg-Gly-Asp三肽。IGF-BP/PP 12基因编码一个1.6 kb的mRNA，在蜕膜、分泌期子宫内膜、肝脏和人肝癌细胞系（HepG 2）中表达。Southern杂交分析表明，在人类基因组中存在单个IGF-BP/PP 12基因。
The low-molecular-mass insulin-like growth factor-binding protein (IGF-BP) and placental protein 12 (PP12) are identical proteins that are present in human serum, amniotic fluid, secretory endometrium and decidua. IGF-BP/PP12 is believed to act as an autocrine or paracrine regulator of cell growth. A cDNA clone encompassing the entire protein coding region of this protein was isolated from a human decidual cDNA library. The authenticity of the cDNA was verified by in vitro transcription/translation experiments and by the identity of the 10 N-terminal amino acids deduced for the mature peptide with those obtained by direct protein sequencing. The amino acid sequence indicates that pre-IGF-BP/PP12 consists of 259 amino acid residues. The putative signal peptide is 25 residues long, and the mature protein thus contains 234 amino acids and has a molecular mass of 25 293 Da. The sequence is very cysteine-rich at the N-terminus after which there are regions of clustered Pro, Glu, Ser and Thr residues (so-called PEST regions), which exist in proteins with short half-lives. The amino acid sequence also includes an Arg-Gly-Asp tripeptide that may function as a cell recognition signal. The IGF-BP/PP12 gene encodes a single 1.6 kb mRNA species that is expressed in decidua, secretory endometrium, liver and a human hepatoma cell line (HepG2). Southern blot analysis suggests that there is a single IGF-BP/PP12 gene in the human genome.