Evidence for a microviscosity increase in the Escherichia coli cell envelope caused by colicin E1.
Evidence for a microviscosity increase in the Escherichia coli cell envelope caused by colicin E1.
复制标题
大肠菌素 E1 引起大肠杆菌细胞包膜微粘度增加的证据。
DOI：
10.1021/bi00712a010
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
F. Lytle
中科院分区：
文献类型：
--
作者：
S. Helgerson;W. Cramer;J. M. Harris;F. Lytle
S. L. Helgerson，W. A. Cramer，* J. M. Harris和FE莱特尔摘要：将大肠杆菌素El以低多重性加入敏感细胞中，导致细胞结合探针A-苯基-1-萘胺的荧光偏振增加。从染料的已知性质和在约16处荧光偏振突变的测量结果推断，染料似乎位于细胞包膜的碳氢化合物区域。在这个温度范围内，具有相似脂肪酸组成的细胞的脂质发生有序-无序转变。由于细胞结合染料的荧光寿命在大肠杆菌素El的存在下也增加，因此得出结论，大肠杆菌素导致染料的旋转弛豫时间增加。以高多重性（10 μ tg/ml）加入的大肠杆菌素El引起荧光偏振从8到26的类似增加，并且不改变有序-无序转变或变化的幅度
S. L. Helgerson, W. A. Cramer,* J. M. Harris, and FE Lytle abstract: Colicin El added at a low multiplicity to sensitive cells causes an increase in the polarization of fluorescence of the cell-bound probe A-phenyl-1-naphthylamine. The dye seems localized in the hydrocarbon regions of the cell envelope as inferred from its known properties and from the measure-ment of an abrupt changein fluorescence polarization at ap-proximately 16. An order-disorder transition in the lipid of cells of similar fatty acid composition has been documented by others to occur in this temperature range. Because the fluores-cence lifetime of the cell-bound dye also increases in the pres-ence of colicin El, it is concluded that colicin causes an in-crease in the rotational relaxation time of the dye. Colicin El added at high multiplicities (10¿ tg/ml) causes a similar in-crease in fluorescence polarization from 8 to 26 and does not alter the magnitude of the order-disorder transition or change