Detailed analysis of inflammatory cell infiltration in colorectal cancer
Detailed analysis of inflammatory cell infiltration in colorectal cancer
复制标题
DOI：
10.1038/bjc.2013.508
复制
发表时间：
2013-10-01
影响因子：
8.8
通讯作者：
Makinen, M. J.
中科院分区：
文献类型：
--
作者：
Vayrynen, J. P.;Tuomisto, A.;Makinen, M. J.
背景：高级别炎性浸润是结直肠癌（CRC）预后较好的一个有前景的标志物。然而，不同的炎症细胞和classification之间的相互关系的知识是fragmental.Methods：我们分析了8种类型的炎症细胞的密度在一个前瞻性招募组的117例CRC患者，并确定其相互关系和贡献Klintrup-Makinen（KM）评分的整体瘤周炎症。我们在24个月的随访中发现了与分期和复发相关的炎性浸润。结果：除了肥大细胞和CD 1a（+）未成熟树突状细胞外，炎性细胞密度之间存在高度正相关。高K-M评分与肿瘤周围和肿瘤内CD 3（+）、CD 8+、CD 68（+）、CD 83+和FoxP 3（+）细胞和中性粒细胞的高密度相关。晚期结直肠癌患者K-M评分低，CD 3+、CD 8+、CD 83+、FoxP 3+细胞计数低，其中K-M评分低、CD 3 + T细胞计数低、FoxP 3 + T细胞计数低与复发率高有关。特别是K-M评分低和T细胞计数低的患者复发率更高。单个炎症标志物之间的高度正相关支持总体炎症反应评分的价值。
Background: Higher-grade inflammatory infiltrate is a promising marker for better prognosis in colorectal cancer (CRC). However, the knowledge on the interrelationships between different inflammatory cells and classifications is fragmentary.Methods: We analysed the densities of eight types of inflammatory cells in a prospectively recruited group of 117 CRC patients and determined their interrelationships and contributions to Klintrup-Makinen (K-M) score of overall peritumoural inflammation. We characterised the inflammatory infiltrate in relation to stage and recurrences in 24-month follow-up.Results: There were high positive correlations between the inflammatory cell densities, with the exception of mast cells and CD1a(+) immature dendritic cells. High K-M score associated with high peri-and intratumoural densities of CD3(+), CD8+, CD68(+), CD83+, and FoxP3(+) cells and neutrophils. Advanced stage associated with low K-M score, as well as low CD3(+), CD8+, CD83(+), and FoxP3(+) cell counts, of which low K-M score, low CD3(+) T-cell count, and low FoxP3(+) T-cell count were linked to higher recurrence rate.Conclusion: The density of CRC inflammatory infiltrate declines as stage advances. Especially, low K-M score and low T-cell counts predict higher recurrence rate. The high positive correlations between the individual inflammatory markers support the value of overall inflammatory reaction scoring.