Design of Biomimetic Vascular Grafts With Magnetic Endothelial Patterning
Design of Biomimetic Vascular Grafts With Magnetic Endothelial Patterning
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DOI：
10.3727/096368912x661300
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发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Wilhelm, Claire
中科院分区：
文献类型：
--
作者：
Fayol, Delphine;Le Visage, Catherine;Wilhelm, Claire
为了有效地进行血管组织工程，仍然需要开发具有可控多细胞组织的小直径血管移植物。在这里，我们描述了一种技术方法相结合的管状支架和磁性标记的细胞，以创建一个多细胞和有组织的血管移植，其内皮化可以通过MRI监测移植前。开发了一种新型支架，其具有管状几何形状和多孔本体结构，使得能够在支架孔中接种细胞。通过用细胞悬液接种冻干支架，获得了人间充质干细胞在大孔结构中的均匀分布。由于实施了基于磁性的图案化技术，因此可以有效地覆盖管的管腔表面。用氧化铁纳米颗粒磁性标记人内皮细胞或内皮祖细胞，并成功地吸引到2 mm的管腔，在那里它们附着并形成连续的内皮。成像方式[荧光成像、组织学和3D磁共振成像（MRI）]的组合证明了血管结构的完整性。特别是，在4.7 T MRI的单细胞分辨率范围内观察血管支架中的不同细胞组织。
The development of small diameter vascular grafts with a controlled pluricellular organization is still needed for effective vascular tissue engineering. Here, we describe a technological approach combining a tubular scaffold and magnetically labeled cells to create a pluricellular and organized vascular graft, the endothelialization of which could be monitored by MRI prior to transplantation. A novel type of scaffold was developed with a tubular geometry and a porous bulk structure enabling the seeding of cells in the scaffold pores. A homogeneous distribution of human mesenchymal stem cells in the macroporous structure was obtained by seeding the freeze-dried scaffold with the cell suspension. The efficient covering of the luminal surface of the tube was then made possible thanks to the implementation of a magnetic-based patterning technique. Human endothelial cells or endothelial progenitors were magnetically labeled with iron oxide nanoparticles and successfully attracted to the 2-mm lumen where they attached and formed a continuous endothelium. The combination of imaging modalities [fluorescence imaging, histology, and 3D magnetic resonance imaging (MRI)] evidenced the integrity of the vascular construct. In particular, the observation of different cell organizations in a vascular scaffold within the range of resolution of single cells by 4.7 T MRI is reported.