Conditional adherence of Enterococcus faecalis to extracellular matrix proteins.
Conditional adherence of Enterococcus faecalis to extracellular matrix proteins.
复制标题
粪肠球菌对细胞外基质蛋白的有条件粘附。
DOI：
10.1111/j.1574-695x.1998.tb01176.x
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发表时间：
1998
影响因子：
--
通讯作者：
Murray,BE
中科院分区：
文献类型：
--
作者：
Xiao,J;Höök,M;Weinstock,GM;Murray,BE
用35S标记的细菌检测了44株心内膜炎常见病原粪肠球菌和13株屎肠球菌对6种细胞外基质（ECM）蛋白底物的粘附性。一个。从心内膜炎患者中分离的粪肠杆菌菌株粘附于I型和IV型胶原蛋白以及另一种胶原蛋白。粪肠杆菌菌株粘附于层粘连蛋白和I型和IV型胶原。然而，当在37°C下生长时，大多数分离株显示出很少的粘附（<5%的添加的细胞粘附），而不管它们的来源（心内膜炎、尿液或粪便样品）。当在46°C下生长时（而不是在CO2或营养限制培养基中生长时），大多数E.粪便增加了它们对固定化层粘连蛋白、I型和IV型胶原的粘附，但对纤维连接蛋白、纤维蛋白原或牛血清白蛋白没有增加，而E. faecium在46°C或50°C下生长时增加粘附。E.通过胰蛋白酶消化消除粪便，表明蛋白质在某种程度上直接或间接地对粘附的发生很重要。用可溶性胶原蛋白I型和IV型预孵育细菌抑制了对这些ECM蛋白的粘附。这些结果表明，在E。粪肠球菌中，对ECM蛋白的粘附是在常规体外生长条件下由偶尔分离物产生的，并且可以在某些应激生长条件下由其他分离物产生。这种坚持是否与E.粪便引起心内膜炎仍有待确定。
The adherence of 44 clinical isolates ofEnterococcus faecalis, a common cause of endocarditis, and 13Enterococcus faeciumto substrates of six extracellular matrix (ECM) proteins was examined using35S-labeled bacteria. OneE. faecalisstrain, isolated from a patient with endocarditis, adhered to collagen types I and IV and anotherE. faecalisstrain adhered to laminin and to collagen types I and IV. However, most isolates showed little adherence (<5% of added cells adhered) when grown at 37°C regardless of their source (endocarditis, urine or fecal sample). When grown at 46°C (but not when grown in CO2or nutrient limited media), most isolates ofE. faecalisincreased their adherence to immobilized laminin, collagen types I and IV but not to fibronectin, fibrinogen or bovine serum albumin, whereas none of theE. faeciumincreased adherence when grown at 46°C or 50°C. The adherence ofE. faecaliswas eliminated by digestion with trypsin, suggesting that a protein is somehow important, directly or indirectly, for adherence to occur. Pre-incubation of bacteria with soluble collagen types I and IV inhibited the adherence to these ECM proteins. These results demonstrate that inE. faecalis, adherence to ECM proteins is produced during routine in vitro growth conditions by occasional isolates and can be produced during certain stressful growth conditions by others. Whether this adherence relates to the propensity ofE. faecalisto cause endocarditis remains to be determined.