Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
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DOI：
10.1007/978-1-61779-523-7_16
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Wilson, Emily
中科院分区：
文献类型：
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作者：
Metz, Richard P.;Patterson, Jan L.;Wilson, Emily
血管平滑肌细胞（Vascular smooth muscle cells, VSMCs）是正常血管壁的细胞组成部分，在血管活性刺激下提供结构完整性并通过动态收缩和舒张调节血管壁直径。VSMC的分化状态以特定的收缩蛋白、离子通道和调节收缩过程的细胞表面受体为特征，因此被称为收缩细胞。除了这些正常功能外，在损伤反应或发育过程中，VSMCs还负责细胞外基质蛋白的合成，变得迁移和增殖。这种表型被称为合成细胞。为了更好地理解调节这些和其他过程的机制，科学家们依赖于可以在体外操纵的培养细胞。在本章中，我们将详细讨论外植体分离VSMC的方法，并将其与酶切法进行比较。我们还将简要描述表征所得细胞的方法。
Vascular smooth muscle cells (VSMCs) are the cellular components of the normal blood vessel wall that provides structural integrity and regulates the diameter by contracting and relaxing dynamically in response to vasoactive stimuli. The differentiated state of the VSMC is characterized by specific contractile proteins, ion channels, and cell surface receptors that regulate the contractile process and are thus termed contractile cells. In addition to these normal functions, in response to injury or during development, VSMCs are responsible for the synthesis of extracellular matrix proteins, become migratory and proliferate. This phenotype has been termed synthetic cells. To better understand the mechanisms regulating these and other processes, scientists have depended on cultured cells that can be manipulated in vitro. In this chapter, we will discuss in detail the explant method for isolation of VSMC and will compare it to the enzymatic digestion method. We will also briefly describe methods for characterizing the resulting cells.