Brain specific human genes, NELL1 and NELL2, are predominantly expressed in neuroblastoma and other embryonal neuroepithelial tumors
Brain specific human genes, NELL1 and NELL2, are predominantly expressed in neuroblastoma and other embryonal neuroepithelial tumors
复制标题
DOI：
10.2176/nmc.41.582
复制
发表时间：
2001-12-01
影响因子：
1.9
通讯作者：
Kuroda, S
中科院分区：
文献类型：
--
作者：
Maeda, K;Matsuhashi, S;Kuroda, S
NELL1和NELL2编码富含半胱氨酸的氨基酸序列，包括6个表皮生长因子样基序，这些基序在n端含有信号肽。两个基因的氨基酸序列有55%相同，半胱氨酸伸展结构保守。NELL1在大脑和肾脏中表达，而NELL2在大脑中特异性表达。通过Northern blot和逆转录聚合酶链式反应分析，研究了神经系统中表达NELLs的细胞系和患者中枢神经系统肿瘤组织。NELL1和NELL2主要在神经母细胞瘤细胞系中表达，在胶质母细胞瘤细胞系中表达较少。NELL1和NELL2在中枢神经细胞瘤、成神经管细胞瘤和一些星形细胞瘤中也有表达。免疫组化分析显示NELL2蛋白定位于神经元细胞质中。这些结果表明，NELL2主要在人类神经系统的神经元细胞系中表达。NELL1主要在肿瘤神经细胞系中表达。
NELL1 and NELL2 encode cysteine-rich amino acid sequences including six epidermal growth factor-like motifs, which contain signal peptides at the N-terminals. The deduced amino acid sequences of both genes are 55% identical and their cysteine stretch structures are conserved. NELL1 is expressed in the brain and kidney, whereas NELL2 is expressed specifically in the brain. The cell lineage expressing NELLs in the nervous system was investigated in established cell lines and central nervous system tumor tissues obtained from patients by Northern blot and reverse transcriptase-polymerase chain reaction analyses. NELL1 and NELL2 were predominantly expressed in neuroblastoma cell lines and little expressed in glioblastoma cell lines. NELL1 and NELL2 were also expressed in central neurocytoma, medulloblastoma, and some astrocytic tumors. Immunohistochemical analysis revealed that NELL2 protein was localized in the cytoplasm of neurons. These results suggest that NELL2 is predominantly expressed in the neuronal cell lineage in the human nervous system. NELL1 is expressed mainly in tumors in the neuronal cell lineage.