β-Cyanoalanine Production by Marine Bacteria on Cyanide-Free Medium and Its Specific Inhibitory Activity toward Cyanobacteria
β-Cyanoalanine Production by Marine Bacteria on Cyanide-Free Medium and Its Specific Inhibitory Activity toward Cyanobacteria
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DOI：
10.1128/aem.66.2.718-722.2000
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发表时间：
2000-02
影响因子：
4.4
通讯作者：
K. Yoshikawa;K. Adachi;M. Nishijima;T. Takadera;Seiji Tamaki;K. Harada;K. Mochida;H. Sano
中科院分区：
文献类型：
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作者：
K. Yoshikawa;K. Adachi;M. Nishijima;T. Takadera;Seiji Tamaki;K. Harada;K. Mochida;H. Sano
摘要 在对雅浦（密克罗尼西亚）、帕劳（Belau）和冲绳（日本西南岛屿）收集的海洋细菌培养液进行抗微藻活性筛选时，发现 2,594 个受测试细菌分离株中的 37 个能够产生针对两栖颤藻 NIES-361 的抗蓝藻物质。选择一种菌株 C-979，被鉴定为弧菌属，并在 2.4 升海洋肉汤 2216 中培养，以鉴定该菌株产生的生物活性化合物。通过仪器分析和先进的 Marfey 方法的应用，纯化的非常亲水的化合物（16.4 mg）被确定为 β-氰基-l-丙氨酸（l-CNAla）。 l-CNAla 不会抑制细菌、酵母或真核微藻的生长，但发现一些蓝细菌在浓度为 0.4 至 25 μg/ml 时对 l-CNAla 敏感。讨论了 l-CNAla 对其他一些环境生物（包括无脊椎动物和大型藻类）的影响。通过薄层色谱法检查了海洋肉汤中 37 种产生抗蓝藻物质的细菌分离株的 CNAla 产量。其中 36 个菌株的肉汤含有 CNAla，表明海洋细菌产生的 CNAla 分布广泛。这是第一份关于在培养基中不提供氰化物离子的情况下产生 CNAl 的细菌的报告。
ABSTRACT In screening the culture broth of marine bacteria collected at Yap (Micronesia), Palau (Belau), and Okinawa (the southwest islands of Japan) for antimicroalgal activity, 37 out of 2,594 bacterial isolates tested were found to produce anticyanobacterial substances against Oscillatoria amphibia NIES-361. One strain, C-979, identified as a Vibrio sp., was selected and cultured in 2.4 liters of marine broth 2216 to identify the bioactive compound produced by the strain. The purified very hydrophilic compound (16.4 mg) was determined to be β-cyano-l-alanine (l-CNAla) by instrumental analyses and the application of the advanced Marfey method. l-CNAla did not inhibit the growth of bacteria, yeast, or eukaryotic microalgae, but some cyanobacteria were found to be sensitive to l-CNAla at a concentration of 0.4 to 25 μg/ml. The effect of l-CNAla on some other environmental organisms, including invertebrates and a macroalgae, is discussed. CNAla production in marine broth was examined by thin-layer chromatography for the 37 bacterial isolates which produced an anticyanobacterial substance. The broth of 36 of these strains contained CNAla, suggesting the wide distribution of CNAla production by marine bacteria. This is the first report on bacteria that produce CNAla without a supply of the cyanide ion in the medium.