Development of a DNA vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination.
Development of a DNA vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination.
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DOI：
10.1016/j.fsi.2004.04.012
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发表时间：
2004-10
影响因子：
4.7
通讯作者：
T. Takano;A. Iwahori;I. Hirono;T. Aoki
中科院分区：
文献类型：
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作者：
T. Takano;A. Iwahori;I. Hirono;T. Aoki
用1 μ g和10 μg编码Hirame弹状病毒（HIRRV）糖蛋白基因的质粒DNA疫苗（pCMV-HRVg）肌肉注射平均体重约2g的牙鲆（Paralichthysolivaceus），发现对HIRRV有较强的保护作用。我们还进行了实时PCR分析，以定量免疫相关基因，例如MHC Iα、IIα、IIβ、TCR-α、β1、β2和δ，以表征DNA疫苗接种后1天和7天的免疫应答。一般来说，拷贝数比未接种疫苗的鱼高至少2倍。有趣的是，TCR β1和β2的基因表达在DNA疫苗接种后1天增加，之后它们的拷贝数恢复到与疫苗接种前相似的水平。这些结果表明，DNA免疫后，比目鱼的免疫系统立即被激活。
Intramuscular injection of Japanese flounder, Paralichthys olivaceus (average weight approximately 2 g) with 1 and 10 μg of a plasmid DNA vaccine encoding the hirame rhabdovirus (HIRRV) glycoprotein gene (pCMV-HRVg) was found to provide strong protection against HIRRV. We also conducted a real-time PCR analysis to quantify immune-related genes, e.g. MHC class Iα, IIα, IIβ, TCR-α, β1, β2 and δ, to characterize the immune response at 1 and 7 days after DNA vaccination. In general, the copy numbers were at least 2-fold higher than those of the non-vaccinated fish. Interestingly, the gene expression of TCR β1 and β2 increased 1 day post-DNA vaccination, after which their copy numbers returned to levels similar to those before vaccination. These results suggest that the immune system of Japanese flounder was activated immediately after DNA immunization.