Onset of C-elegans gastrulation is blocked by inhibition of embryonic transcription with an RNA polymerase antisense RNA
Onset of C-elegans gastrulation is blocked by inhibition of embryonic transcription with an RNA polymerase antisense RNA
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DOI：
10.1006/dbio.1996.0232
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发表时间：
1996-09-15
影响因子：
2.7
通讯作者：
Wood, WB
中科院分区：
文献类型：
--
作者：
PowellCoffman, JA;Knight, J;Wood, WB
秀丽隐杆线虫胚胎中的卵裂和原肠胚形成的特征是细胞分裂和细胞运动的不变的时间和空间模式。尽管大量胚胎转录直到原肠胚形成才开始，但一些转录早在 4 细胞阶段就可以检测到。为了确定正常卵裂阶段模式是否需要任何早期转录本，我们通过向雌雄同体亲本性腺注射ama-1基因的RNA反义RNA来阻断胚胎中的转录，ama-1基因编码RNA聚合酶II的大亚基。这种处理没有阻止由早期胚胎启动子驱动的报告基因的表达，但没有可检测地扰乱种系P颗粒的母体控制分离或通过前四次分裂的细胞分裂模式。然而，在第五个细胞周期中，两个内胚层前体 (E) 细胞早期异常分裂，无法启动原肠胚形成。胚胎在第六和第七个细胞周期之间停滞，细胞数少于 100 个。这些结果表明胚胎转录的基因产物是原肠胚形成起始所必需的。他们还证明了一种阻断胚胎转录的方法的功效，该方法可能对其他生物体有用。 (C) 1996 学术出版社
Cleavage and gastrulation initiation in Caenorhabditis elegans embryos are characterized by an invariant temporal and spatial pattern of cell divisions and cell movements. Although bulk embryonic transcription does not begin until gastrulation onset, some transcription can be detected as early as the 4-cell stage. To determine whether any early transcripts are required for normal cleavage-stage patterning, we blocked transcription in embryos by injecting hermaphrodite parental gonads with RNA antisense to the ama-1 gene, which encodes the large subunit of RNA polymerase II. This treatment no prevented the expression of a reporter gene driven by an early embryonic promoter but did not detectably perturb the maternally controlled segregation of the germ line P granules or the pattern of cell division through the first four cleavages. in the fifth cell cycle, however, the two endodermal precursor (E) cells divided early and abnormally and failed to initiate gastrulation. The embryos arrested between the sixth and seventh cell cycles with less than 100 cells. These results indicate that embryonically transcribed gene products are required for gastrulation initiation. They also demonstrate the efficacy of a method for blocking embryonic transcription that may be useful in other organisms. (C) 1996 Academic Press, Inc.