Functional analysis of female-biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze)
Functional analysis of female-biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze)
复制标题
Adelphocoris lineolatus (Goeze) 中雌性气味结合蛋白 6 对挥发性和非挥发性宿主化合物的功能分析
DOI：
10.1111/imb.12322
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发表时间：
2017-10-01
影响因子：
2.6
通讯作者：
Guo, Y. -Y.
中科院分区：
文献类型：
--
作者：
Sun, L.;Wang, Q.;Guo, Y. -Y.
多食性蝽 Adelphocoris lineolatus 严重依赖嗅觉线索来追踪合适的寄主植物。因此，更好地了解其嗅觉检测的分子基础可能有助于制定有效的害虫管理策略。在本研究中，我们报告了气味结合蛋白基因 A.lineolatus 气味结合蛋白 6 (AlinOBP6) 的表达谱。定量实时 PCR 实验表明 AlinOBP6 是雌性成虫触角偏向的。细胞免疫定位分析表明，AlinOBP6 在多孔基本感器和单孔感器的淋巴中高表达。配体结合分析表明，重组AlinOBP6不仅与宿主植物挥发性化合物紧密结合，而且与非挥发性化合物紧密结合。同源建模和分子对接分析证实了这些不寻常的配体结合特征，并揭示了参与识别挥发性和非挥发性化合物的氨基酸残基是不同的。我们的研究结果首次表明半翅目昆虫中触角和雌性偏向的 OBP 在嗅觉和味觉中表达，作为对挥发性和非挥发性宿主化合物做出反应的机制。这些发现值得进一步研究蝽响应宿主植物位置的化学感受的分子机制。
The polyphagous mirid bug Adelphocoris lineolatus relies heavily on olfactory cues to track suitable host plants. Thus, a better understanding of the molecular basis of its olfactory detection could contribute to the development of effective pest management strategies. In the present study, we report the expression profile of the odorant binding protein gene A.lineolatus odorant binding protein 6 (AlinOBP6). Quantitative real-time PCR experiments suggest that AlinOBP6 is female adult antennae-biased. Cellular immunolocalization analyses show that AlinOBP6 is highly expressed in the lymph of both multiporous sensilla basiconica and uniporous sensilla chaetica. A ligand binding analysis showed that recombinant AlinOBP6 not only bound tightly to host plant volatile compounds but also to nonvolatile compounds. Homology modelling and molecular docking analyses confirmed these unusual ligand binding profiles and revealed that the amino acid residues involved in the recognition of volatile and nonvolatile compounds are distinct. The results of our study are the first to suggest that an antenna- and female-biased OBP in an hemipteran insect is expressed in both olfactory and gustatory sensilla as a mechanism to respond to volatile and nonvolatile host compounds. These findings warrant further research into the molecular mechanisms of chemosensation for mirid bugs in responsive to host plant location.