Characterization of Salmonella enterica isolates from infants and toddlers in Wuhan, China
Characterization of Salmonella enterica isolates from infants and toddlers in Wuhan, China
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中国武汉婴幼儿肠道沙门氏菌分离株的特征
DOI：
10.1093/jac/dkn452
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发表时间：
2009-01-01
影响因子：
5.2
通讯作者：
Ma, Yue
中科院分区：
文献类型：
--
作者：
Cui, Shenghui;Li, Jingyun;Ma, Yue
超广谱头孢菌素类和氟喹诺酮类是治疗侵袭性沙门氏菌病的重要抗菌药物，对这些抗菌药物的耐药性是最重要的问题。对2006年从0 ~ 3岁儿童门诊分离的肠道沙门氏菌进行血清学分型、药敏试验、β-内酰胺酶基因、喹诺酮类药物耐药决定区（QRDRs）突变、qnr等位基因和aac-（6 '）-Ib-cr突变的PCR检测及DNA序列分析。所有鼠伤寒沙门氏菌分离株和43株非鼠伤寒沙门氏菌分离株进一步用脉冲场凝胶电泳进行鉴定，以确定遗传相关性。回收了29种不同血清型的肠道分离株。不同血清型的抗菌素耐药性不同。环丙沙星耐药分离株集中在对至少4种其他非喹诺酮类抗生素耐药的鼠伤寒血清型中。将22株耐环丙沙星鼠伤寒沙门氏菌分离株中的19株分为一个PFGE簇。18株S.肠道分离物和4株分离物也携带qnr等位基因。在7株头孢曲松耐药的鼠伤寒沙门氏菌中发现了质粒介导的bla（CTX-M-14）样基因，其中2株对环丙沙星的敏感性降低，提示氟喹诺酮类药物不应用于本地区侵袭性鼠伤寒沙门氏菌感染的治疗。监测计划应保持警惕，头孢曲松耐药的S。肠道分离株对氟喹诺酮敏感性降低。
Extended-spectrum cephalosporins and fluoroquinolones are important antimicrobials for treating invasive salmonellosis, and emerging resistance to these antimicrobials is of paramount concern. This study reports on the antimicrobial susceptibility and molecular characterization of Salmonella enterica isolates recovered in 2006 from 0- to 3-year-old outpatients in China.The isolates were subjected to serotyping, antimicrobial susceptibility testing, screening for beta-lactamase genes, mutations in the quinolone resistance determining regions (QRDRs), qnr alleles and aac-(6')-Ib-cr by PCR followed by DNA sequence analysis. All Salmonella Typhimurium isolates and 43 selected non-Typhimurium isolates were further characterized by PFGE to determine the genetic relatedness.From 3746 paediatric outpatient stool samples, 221 (5.9%) S. enterica isolates of 29 distinct serotypes were recovered. The antimicrobial resistance profiles differed among serotypes. Ciprofloxacin-resistant isolates were concentrated in serotype Typhimurium that were resistant to at least four additional non-quinolone antimicrobials. Nineteen out of 22 ciprofloxacin-resistant Salmonella Typhimurium isolates were grouped into one PFGE cluster. Plasmid-mediated quinolone resistance determinant aac-(6')-Ib-cr was detected in 18 S. enterica isolates and 4 isolates also carried qnr alleles. Plasmid-mediated bla(CTX-M-14)-like genes were found in seven ceftriaxone-resistant isolates, and two isolates also exhibited reduced susceptibility to ciprofloxacin.Based on these results, fluoroquinolones should not be used to treat the invasive Salmonella Typhimurium infections in this local community. The monitoring programme should stay vigilant for ceftriaxone-resistant S. enterica isolates with reduced fluoroquinolone susceptibility.