Expression of RGS2 alters the coupling of metabotropic glutamate receptor 1a to M-type K+ and N-type Ca2+ channels
Expression of RGS2 alters the coupling of metabotropic glutamate receptor 1a to M-type K+ and N-type Ca2+ channels
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DOI：
10.1016/s0896-6273(00)80740-0
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发表时间：
1999-04-01
期刊：
影响因子：
16.2
通讯作者：
Ikeda, SR
中科院分区：
文献类型：
--
作者：
Kammermeier, PJ;Ikeda, SR
交感神经元异源表达的I组mGluRs抑制钙电流(I-Ca)和M型钾电流(I-M)。经百日咳毒素(PTX)处理后，发现电压依赖性(VD)、PTX敏感的钙抑制成分和电压非依赖性(VI)、PTX不敏感的成分。在PTX处理的细胞中，RGS2共表达阻断mGluR1a对I-M的抑制作用，并使I-Ca抑制VD，可能是通过阻断Gα(Q/11)-GTP的作用。这些数据表明，mGluR1a可以与G(I/O)和G(Q/11)偶联。此外，VI I-Ca抑制是通过Gα(Q/11)-GTP介导的途径进行的，该途径可以通过表达RGS2来阻断，使VD，Gβ伽马介导的抑制处于激活状态。这些数据可能揭示了体内系统中RGS2表达上调的功能作用。
Group I mGluRs heterologously expressed in sympathetic neurons inhibited calcium (I-Ca) and M-type potassium (I-M) currents. Treatment with pertussis toxin (PTX) revealed a voltage-dependent (VD), PTX-sensitive component of I-Ca inhibition and a voltage-independent (VI), PTX-insensitive component. Coexpression of RGS2 occluded mGluR1a inhibition of I-M and made I-Ca inhibition VD in PTX-treated cells, presumably by blocking the effects of G alpha(q/11)-GTP. These data indicate that mGluR1a can couple to G(i/o) as well as G(q/11). In addition, VI I-Ca inhibition proceeds through a G alpha(q/11)-GTP-mediated pathway, which can be occluded by expressing RGS2, leaving the VD, G beta gamma-mediated inhibition active. These data may reveal a functional role for the upregulation of RGS2 expression in in vivo systems.