Microrobotic Streak Seeding For Protein Crystal Growth
Microrobotic Streak Seeding For Protein Crystal Growth
复制标题
用于蛋白质晶体生长的微型机器人条纹播种
DOI：
10.15607/rss.2005.i.019
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发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
John E. Hunt
中科院分区：
文献类型：
--
作者：
A. Gueorguiev;P. Allen;Ting Song;A. Laine;W. Edstrom;John E. Hunt
我们提出了一种用于蛋白质晶体微操作任务的微机器人系统。本文的重点是晶体学家称为条纹播种的任务-它用于吸引某些蛋白质晶体生长。我们的系统具有一套定制设计的微定位器末端执行器，我们称之为微铲，以取代晶体学家用于这项任务的传统工具。我们使用微机电系统（MEMS）技术设计和制造各种形状和数量的微型铲。来自安装在显微镜上的摄像机的视觉输入用于检测工具需要接触的源晶体的位置以及用于接种的靶蛋白液滴的位置。我们目前的实验结果，说明我们的方法的适用性。
We present a microrobotic system for protein crystal micromanipulation tasks. The focus in this paper is on the task known to crystallographers as streak seeding – it is used to entice certain protein crystals to grow. Our system features a set of custom designed micropositioner end-effectors we call microshovels to replace traditional tools used by crystallographers for this task. We have used micro-electrical mechanical system (MEMS) techniques to design and manufacture various shapes and quantities of microshovels. Visual input from a camera mounted on the microscope is used to detect the locations of the source crystals which the tool needs to touch as well as the locations of the target protein droplets for seeding. We present experimental results that illustrate the applicability of our approach.