Development of Dual Neurotrophins-Encapsulated Electrospun Nanofibrous Scaffolds for Peripheral Nerve Regeneration
Development of Dual Neurotrophins-Encapsulated Electrospun Nanofibrous Scaffolds for Peripheral Nerve Regeneration
复制标题
用于周围神经再生的双神经营养素封装电纺纳米纤维支架的开发
DOI：
10.1166/jbn.2016.2299
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发表时间：
2016
影响因子：
2.9
通讯作者：
Mo Xiumei
中科院分区：
文献类型：
--
作者：
Sun Binbin;Wu Tong;He Liping;Zhang Jianguang;Yuan Yuyu;Huang Xijun;EI-Hamshary Hany;Al-Deyab Salem S.;Xu Tao;Mo Xiumei
Nerve growth factor (NGF) is widely used for repairing peripheral nerve injury because of its capability in dominating the survival, migration, proliferation, and differentiation of nerve cells. Monosialoganglioside (GM1), as another kind of nerve growth factor, works for regulating NGF function. In this study, GM1 and NGF were incorporated into the Poly(l-lactic acid-co-ε-caprolactone)/silk fibroin (PLCL/SF) nanofibers by the coaxial electrospinning. The fibers morphology and core–shell structure were characterized by SEM and TEM. The scaffolds demonstrated high tensile stress with good flexibility. In vitro cell viability studies indicated that the scaffolds incorporating both GM1 and NGF played a synergistic effect to enhance Schwann cells (SCs) proliferation and Pheochromocytoma (PC12) cells differentiation, in comparison to the scaffolds only incorporating NGF. Subsequently, the nanofibrous conduit scaffolds (NCSs) were evaluated in vivo in a rabbit sciatic nerve defect model. The NGF/GM1 incorporated NCSs group performed better nerve function recovery than single incorporated group, in consideration of the compound muscle action potential (CMAP) and nerve conduction velocity (NCV) results. Furthermore, hematoxylin and eosin (H&E) staining, toluidine blue (TB) staining, and transmission electron microscope (TEM) analysis displayed better nerve regeneration of NGF/GM1 incorporated NCSs both quantitatively and qualitatively. Therefore, the results indicated the dual neurotrophins-incorporated NCSs had potentials for the application in peripheral nerve repairing.
