Sector analysis reveals patterns of cambium differentiation in poplar stems
Sector analysis reveals patterns of cambium differentiation in poplar stems
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DOI：
10.1093/jxb/ery230
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发表时间：
2018-08-17
影响因子：
6.9
通讯作者：
Spokevicius, Antanas V.
中科院分区：
文献类型：
--
作者：
Bossinger, Gerd;Spokevicius, Antanas V.
我们使用扇区分析来研究形成层的发育和动态，并测试形成层原始细胞作为真正的“干细胞”和更多分化的母细胞之间是否存在根本的发育和功能差异。在许多高等植物中，沿植物中轴形成一个圆柱形的侧生分生组织，即维管形成层。最明显的是在多年生树种的茎中，这种分生组织向树干内部形成木质部(木材)，向外形成韧皮部(树皮)。因此，维管形成层负责地球上大部分森林生物量的生产，对全球碳循环做出了重大贡献。利用农杆菌介导的杨树体内茎转化实验中的细菌uidA报告基因，我们创建了379个起源于单个细胞转化的形成层区段。我们对扇形频率和模式的分析结果与杨树形成层的特征一致，形成层只有一层真正的形成层首字母(能够区分反形成层和周向形成层)。我们发现，形成层中的原始细胞经常丢失，这种细胞丢失很少发生在母细胞水平，韧皮部和木质部的分化是独立控制的，母细胞补充的频率不是预先确定的。
We used sector analysis to study cambium development and dynamics and to test whether fundamental developmental and functional differences exist between cambial initials as true ` stem cells' and more differentiated mother cells. In many higher plants, a cylindrical lateral meristem, the vascular cambium, forms along the plant axis. Most notably in stems of perennial tree species, this meristem gives rise to xylem (wood) towards the inside of the trunk and phloem (bark) towards the outside. As such, the vascular cambium is responsible for the production of most of the planet's forest biomass, significantly contributing to the global carbon cycle. Using the bacterial uidA reporter gene in Agrobacterium-based in vivo stem transformation experiments in poplar trees, we created 379 cambium sectors that originated from the transformation of individual cells. Results from our analysis of sector frequency and patterns are consistent with the poplar cambium featuring a single layer of true cambial initials (being able to divide both anti-and periclinally). We show that initials are frequently lost from the cambium, that such cell loss rarely occurs at mother cell level, that phloem and xylem differentiation are controlled independently, and that the frequency of mother cell replenishment is not pre-determined.