Origin of the tetraspanin uroplakins and their co-evolution with associated proteins:: Implications for uroplakin structure and function
Origin of the tetraspanin uroplakins and their co-evolution with associated proteins:: Implications for uroplakin structure and function
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DOI：
10.1016/j.ympev.2006.04.023
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发表时间：
2006-11-01
影响因子：
4.1
通讯作者：
DeSalle, Rob
中科院分区：
文献类型：
--
作者：
Garcia-Espana, Antonio;Chung, Pei-Jung;DeSalle, Rob
基因组水平的信息与特定基因和基因家族的系统发育分析相结合，可以更好地了解其蛋白质产物的结构和功能。在这项研究中，我们使用来自全基因组序列的系统发育方法，研究了哺乳动物四跨蛋白超家族成员，分别与uroplakins UPII和UPIIIa/IIIb相互作用的uroplakins Ia和Ib。这些蛋白相互作用形成尿路上皮斑块，在膀胱顶端尿路上皮表面的渗透性屏障功能中起核心作用。由于这些斑块仅在哺乳动物尿路上皮中发现，因此最近在没有典型尿路上皮的低等脊椎动物中发现up样基因组序列是令人费解的。我们克隆了来自青蛙（Xenopus laevis）、鸡（Gallus Gallus）和斑马鱼（Danio rerio）的全长up相关cdna，并将这些数据与所有可用的完全测序和注释的动物基因组中的同源物序列信息相结合。对所有可用的uroplakin序列进行系统发育分析，并对其在若干动物分类群中的分布进行了解，结果表明：(i) UPIa/UPIb和UPII/UPIII基因在脊椎动物共同祖先中通过基因复制进化而来；（ii）尾白蛋白在脊椎动物谱系中可能以不同的组合丢失；（iii）在UPIa和UPIb及其伙伴UPII和UPIIIa/IIIb之间存在很强的共同进化关系。四跨蛋白UPs及其相关蛋白的共同进化可能会微调uroplakin复合物的结构和功能，使其能够执行多种物种和组织特异性功能。在爪蟾的肾脏、卵母细胞和脂肪体中也有表达的uroplakins，其结构和功能比目前所认识的要复杂得多。(c) 2006爱思唯尔公司版权所有。
Genome level information coupled with phylogenetic analysis of specific genes and gene families allow for a better understanding of the structure and function of their protein products. In this study, we examine the mammalian uroplakins (UPs) Ia and Ib, members of the tetraspanin superfamily, that interact with uroplakins UPII and UPIIIa/IIIb, respectively, using a phylogenetic approach of these genes from whole genome sequences. These proteins interact to form urothelial plaques that play a central role in the permeability barrier function of the apical urothelial surface of the urinary bladder. Since these plaques are found exclusively in mammalian urothelium, it is enigmatic that UP-like genomic sequences were recently found in lower vertebrates without a typical urothelium. We have cloned full-length UP-related cDNAs from frog (Xenopus laevis), chicken (Gallus gallus), and zebrafish (Danio rerio), and combined these data with sequence information from their orthologs in all the available fully sequenced and annotated animal genomes. Phylogenetic analyses of all the available uroplakin sequences, and an understanding of their distribution in several animal taxa, suggest that: (i) the UPIa/UPIb and UPII/UPIII genes evolved by gene duplication in the common ancestor of vertebrates; (ii) uroplakins can be lost in different combinations in vertebrate lineages; and (iii) there is a strong co-evolutionary relationship between UPIa and UPIb and their partners UPII and UPIIIa/IIIb, respectively. The co-evolution of the tetraspanin UPs and their associated proteins may fine-tune the structure and function of uroplakin complexes enabling them to perform diverse species- and tissue-specific functions. The structure and function of uroplakins, which are also expressed in Xenopus kidney, oocytes and fat body, are much more versatile than hitherto appreciated. (c) 2006 Elsevier Inc. All rights reserved.