SOLUBLE FORMS OF E-SELECTIN, ICAM-1 AND VCAM-1 ARE PRESENT IN THE SUPERNATANTS OF CYTOKINE ACTIVATED CULTURED ENDOTHELIAL-CELLS
SOLUBLE FORMS OF E-SELECTIN, ICAM-1 AND VCAM-1 ARE PRESENT IN THE SUPERNATANTS OF CYTOKINE ACTIVATED CULTURED ENDOTHELIAL-CELLS
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DOI：
10.1016/0006-291x(92)91234-h
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发表时间：
1992-09-16
影响因子：
3.1
通讯作者：
GEARING, AJH
中科院分区：
文献类型：
--
作者：
PIGOTT, R;DILLON, LP;GEARING, AJH
在细胞因子激活的培养内皮细胞的上清液中检测到可溶形式的粘附分子ICAM-1、VCAM-1和E-选择素。已使用双位点酶免疫测定法定量释放。释放的分子的分子量已通过免疫沉淀法测定，并且与通过在靠近膜插入点的位点处裂解产生可溶形式一致。
Soluble forms of the adhesion molecules ICAM-1, VCAM-1 and E-selectin have been detected in supernatants from cytokine activated cultured endothelial cells. The release has been quantified using two site enzyme immunoassays. The molecular weights of the released molecules have been determined by immunoprecipitation and are consistent with the generation of soluble forms by cleavage at a site close to the point of membrane insertion.