Genes encoding general initiation factors for RNA polymerase II transcription are dispersed in the human genome.
Genes encoding general initiation factors for RNA polymerase II transcription are dispersed in the human genome.
复制标题
编码 RNA 聚合酶 II 转录通用起始因子的基因分散在人类基因组中。
DOI：
10.1093/hmg/3.1.61
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发表时间：
1994
影响因子：
3.5
通讯作者：
L. Tsui
中科院分区：
文献类型：
--
作者：
H. Heng;H. Xiao;X. Shi;J. Greenblatt;L. Tsui
一般转录因子是RNA聚合酶II准确启动转录所必需的，人类编码这些转录因子亚单位的cDNAs已被克隆和测序。利用荧光原位杂交技术，我们发现编码TFIIH TATA盒结合蛋白(TBP)、TFIIB、TFIIEα、TFIIEβ、RAP30、RAP74和62 kDa亚基的基因分别位于人类染色体6q26-27、1p21-22、3q21-24、8p12、13q14、19p13.3和11p14-15.1带。这种分散的一组功能相关基因的定位为深入了解人类基因组进化的分子机制及其可能参与人类疾病提供了洞察力。
General transcription factors are required for accurate initiation of transcription by RNA polymerase II. Human cDNAs encoding subunits of these factors have been cloned and sequenced. Using fluorescence in situ hybridization (FISH), we show here that the genes encoding the TATA-box binding protein (TBP), TFIIB, TFIIE alpha, TFIIE beta, RAP30, RAP74 and the 62 kDa subunit, of TFIIH are located at the human chromosomal bands 6q26-27, 1p21-22, 3q21-24, 8p12, 13q14, 19p13.3 and 11p14-15.1, respectively. This dispersed localization of a group of functionally related gene provides insights into the molecular mechanism of human genome evolution and their possible involvement in human diseases.