Experimental studies on anti-tumor effects of Capilliposide
Experimental studies on anti-tumor effects of Capilliposide
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DOI：
10.3969/j.issn.1001-1978.2012.04.024
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发表时间：
2012-04-01
期刊：
影响因子：
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通讯作者：
Tian Jing-kui
中科院分区：
文献类型：
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作者：
Xu Yan;Rong Yu-mei;Tian Jing-kui
目的研究毛细菌素(LC)对裸鼠和C57BL/6肿瘤模型的抗肿瘤作用，并探讨其对免疫系统的调节作用。方法建立荷人肿瘤裸鼠模型。然后将模型分为不同的组。在给药期间记录体重和肿瘤体积。用免疫组织化学方法检测肿瘤组织的组织病理学和血管内皮生长因子的表达。建立了携带RM-1的C57BL/6小鼠模型。检测LC对C57BL/6小鼠模型的抗肿瘤作用。检测血液学指标、脾T淋巴细胞增殖反应和IL-2活性，评价其对免疫功能的影响。结果LC能有效抑制肿瘤生长，并呈剂量依赖关系。最高抑制率PC-3为58.77%，BGC-823为56.22%，SK-OV-3为55.73%。LC能上调RM-1荷瘤小鼠外周血中WBC和HGB的含量。能显著提高小鼠脾T淋巴细胞增殖活性和IL-2活性。结论LC具有较强的抗肿瘤活性。其作用机制可能与提高免疫功能、下调血管内皮生长因子有关。
Aim To study the anti-tumor effect of Capilliposide (LC) in nude mice and C57BL/6 models and to evaluate its regulation of immune system. Methods Models of nude mice bearing human cancers were established. Then the models were divided into different groups. The body weight and tumor volume were recorded during the period of administration. The histopathology and VEGF of tumor tissue was measured by immunohistochemistry. Models of C57BL/6 mice bearing RM-1 were established. The anti-tumor effect of LC on C57BL/6 mice model was detected. Then hematological parameters, proliferation of spleen T lymphocyte and IL-2 activity were measured to evaluate its effects on immunity. Results LC could effectively inhibit tumor growth in a dose-dependent mode. The highest inhibitory rate was 58.77% ( PC-3), 56.22% (BGC-823) and 55.73% ( SK-OV-3) separately. LC could up-regulate WBC and HGB in peripheral blood of mice bearing RM-1. It significantly increased proliferation of spleen T lymphocyte and IL-2 activity. Conclusions It clearly suggests that LC has strong antitumor activity. Its effect may depend on immune improving and VEGF down-regulation.