Genital chlamydial infection: association between clinical features, organism genotype and load
Genital chlamydial infection: association between clinical features, organism genotype and load
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DOI：
10.1099/jmm.0.028076-0
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发表时间：
2011-07-01
影响因子：
3
通讯作者：
Sonnex, Christopher
中科院分区：
文献类型：
--
作者：
Jalal, Hamid;Verlander, Neville Q.;Sonnex, Christopher
生殖道衣原体感染的临床特征与微生物基因型和载量之间的关系进行了评估。在泌尿生殖医学诊所就诊的3384名连续患者中，有233名（7%）的生殖器拭子中检测到并定量了衣原体DNA。衣原体阳性亚组包括132名（57%）女性和101名（43%）男性。33%的女性和72%的男性存在临床特征。发现女性的衣原体负荷（中位数负荷：5.6 log）高于男性（中位数负荷：3.5 log）。单变量分析未能显示衣原体载量与临床特征之间的显著相关性（P值= 0.3）。由于临床资料有限，70%（n = 162）的衣原体阳性患者可获得衣原体基因型信息。然而，这些样本的多变量分析显示衣原体载量和临床特征之间存在显著相关性（P值= 0.02）。这种差异很可能是由于单变量（233例患者的数据）和多变量（162例患者的数据）分析的数据量不同。衣原体基因型分布依次为E型（46%）、F型（22%）、D型（8%）、K型（8%）、G型（7%）、J型（4%）、I型（1%）和H型（0.6%）。在单变量（P值= 0.6）或多变量（P值= 0.4）分析中，未观察到衣原体基因型与临床特征之间存在统计学显著相关性。这些结果表明，衣原体负载和ompA基因的多样性在生殖器衣原体感染的发病机制中起很小的作用，如果有的话。
The association between the clinical features of genital chlamydial infection and organism genotype and load was evaluated. Chlamydial DNA was detected and quantified in genital swabs from 233 (7 %) of 3384 consecutive patients attending a genitourinary medicine clinic. The chlamydia-positive subcohort comprised 132 (57%) females and 101 (43%) males. Clinical features were present in 33% women and 72% men. The chlamydial load was found to be higher in women (median load: 5.6 log) than men (median load: 3.5 log). Single variable analysis failed to show a significant association between chlamydial load and clinical features (P value = 0.3). Owing to the limited amount of clinical material, information on chlamydial genotypes was available for 70% (n = 162) of chlamydia-positive patients. However, multivariable analysis of these samples did show a significant association between chlamydial load and clinical features (P value = 0.02). This discrepancy is most probably due to the difference in the amount of data analysed by single variable (data from 233 patients) and multivariable (data from 162 patients) analysis. The distribution of chlamydial genotypes was as follows: type E (46%), F (22%), D (8 %), K (8 %), G (7 %), J (4 %), I (1%) and H (0.6%). No statistically significant association was observed between chlamydial genotype and clinical features in either single variable (P value = 0.6) or multivariable (P value = 0.4) analysis. These findings suggest that chlamydial load and diversity in the ompA gene plays little, if any, role in the pathogenesis of genital chlamydial infection.