Isolation and characterization of the alkaline protease gene of Aspergillus oryzae.
Isolation and characterization of the alkaline protease gene of Aspergillus oryzae.
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米曲霉碱性蛋白酶基因的分离和表征。
DOI：
10.1080/00021369.1991.10871048
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Hirofumi Arimura
中科院分区：
文献类型：
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作者：
K. Murakami;Yutaka Ishida;A. Masaki;Hiroki Tatsumi;Seiji Murakami;E. Nakano;H. Motai;Haruhide Kawabe;Hirofumi Arimura
利用人工合成的寡核苷酸作为杂交探针，提取了真菌米曲霉碱性蛋白酶(ALP)的基因组DNA，并对其全序列进行了鉴定。ALP基因全长1374个核苷酸，包含三个内含子，其中一个在PRO区，两个在成熟编码区。在5‘-非编码区发现了与TATA盒(TATAAAT)和CAAT盒(CCAAAT)相关的序列。引物延伸分析表明，存在3个转录起点。
The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAAT box (CCAAAT) were found in the 5'-noncoding region. Primer extension analysis showed that three transcriptional start points are present.