Expansions and contractions in a tandem repeat induced by double-strand break repair
Expansions and contractions in a tandem repeat induced by double-strand break repair
复制标题
DOI：
10.1128/mcb.18.4.2045
复制
发表时间：
1998-04-01
影响因子：
5.3
通讯作者：
Haber, JE
中科院分区：
文献类型：
--
作者：
Pâques, F;Leung, WY;Haber, JE
酵母中双链断裂(DSB)的修复可以在375个碱基的重复序列中诱导非常频繁的扩张和收缩。这些结果有力地表明，DSB修复可能是重复序列扩增的一个主要来源。大多数DSB维修事件与交叉无关。在这些修复的受体分子中，有50%出现重排。相比之下，供体模板几乎总是保持不变。在罕见的交叉事件中，也发现了类似的重排。这些结果不能很容易地用Szostak等人的缺口修复模型解释。(J.W.Szostak，T.L.Orr-Weaver，R.J.Rothstein和F.W.Stahl，Cell 33：25-35,1983)，但可以用允许交叉的合成依赖链退火(SDSA)模型来解释。对SDSA模型的支持是由一个DSB修复事件可以使用位于两个不同染色体上的两个供体模板的演示提供的。
Repair of a double-strand break (DSB) in yeast can induce very frequent expansions and contractions in a tandem array of 375-bp repeats. These results strongly suggest that DSB repair can be a major source of amplification of tandemly repeated sequences. Most of the DSB repair events are not associated with crossover. Rearrangements appear in 50% of these repaired recipient molecules. In contrast, the donor template nearly always remains unchanged. Among the rare crossover events, similar rearrangements are found. These results cannot readily be explained by the gap repair model of Szostak et al. (J. W. Szostak, T. L. Orr-Weaver, R. J. Rothstein, and F. W. Stahl, Cell 33:25-35, 1983) but can be explained by synthesis-dependent strand annealing (SDSA) models that allow for crossover. Support for SDSA models is provided by a demonstration that a single DSB repair event can use two donor templates located on two different chromosomes.