Accelerated differentiation of neural stem cells into neurons on ginseng-reduced graphene oxide sheets
Accelerated differentiation of neural stem cells into neurons on ginseng-reduced graphene oxide sheets
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DOI：
10.1016/j.carbon.2013.09.015
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发表时间：
2014-01-01
期刊：
影响因子：
10.9
通讯作者：
Ghasemi, Effat
中科院分区：
文献类型：
--
作者：
Akhavan, Omid;Ghaderi, Elham;Ghasemi, Effat
使用亚洲红参将化学剥离的氧化石墨烯（GO）绿色还原成还原的氧化石墨烯（rGO）。人参-rGO片的还原水平和电导率与肼-rGO片的还原水平和电导率相当。人参还原导致rGO的sp（2）石墨结构的修复，而肼-rGO显示出更多的缺陷和/或更小的芳香域。人参-rGO片呈现出比肼-rGO片在水悬浮液中更好的抗聚集稳定性。虽然疏水性肼-rGO膜对人神经干细胞（hNSC）没有表现出毒性，但亲水性GO和人参-rGO膜（作为生物相容性更好的膜）在3天后显示出干细胞的增殖。另一方面，肼-rGO和特别是人参-rGO膜在3周后比GO膜表现出更多的hNSC向神经元（而不是神经胶质）的分化。rGO膜上的加速分化归因于其更高的电子转移能力。同时，人参-rGO膜（与肼-rGO膜相比）上的更好的分化归因于其更高的生物相容性、更大的亲水性和还原片表面上的抗氧化剂分子（作为强力抗氧化剂）的π-π附着。(C)2013爱思唯尔有限公司保留所有权利。
Asian red ginseng was used for green reduction of chemically exfoliated graphene oxide (GO) into reduced graphene oxide (rGO). The reduction level and electrical conductivity of the ginseng-rGO sheets were comparable to those of hydrazine-rGO ones. Reduction by ginseng resulted in repairing the sp(2) graphitic structure of the rGO, while hydrazine-rGO showed more defects and/or smaller aromatic domains. The ginseng-rGO sheets presented a better stability against aggregation than the hydrazine-rGO ones in an aqueous suspension. Whilst the hydrophobic hydrazine-rGO films exhibited no toxicity against human neural stem cells (hNSCs), the hydrophilic GO and ginseng-rGO films (as more biocompatible films) showed proliferation of the stem cells after 3 days. On the other hand, the hydrazine-rGO and especially the ginseng-rGO films exhibited more differentiation of hNSCs into neurons (rather than glia) than the GO film, after 3 weeks. The accelerated differentiation on the rGO films was assigned to their higher capability for electron transfer. Meanwhile, the better differentiation on the ginseng-rGO film (as compared to the hydrazine-rGO film) was attributed to its higher biocompatibility, more hydrophilicity and the pi-pi attachment of ginsenoside molecules (as powerful antioxidants) on surface of the reduced sheets. (C) 2013 Elsevier Ltd. All rights reserved.