Yerba mate (Ilex paraguariensis) enhances the gene modulation and activity of paraoxonase-2: In vitro and in vivo studies
Yerba mate (Ilex paraguariensis) enhances the gene modulation and activity of paraoxonase-2: In vitro and in vivo studies
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DOI：
10.1016/j.nut.2012.04.011
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发表时间：
2012-11-01
期刊：
影响因子：
4.4
通讯作者：
da Silva, Edson Luiz
中科院分区：
文献类型：
--
作者：
Fernandes, Elenise Stucker;Machado, Marcos de Oliveira;da Silva, Edson Luiz
目的：对氧磷酶-2（PON-2）是一种细胞内的抗氧化酶，多酚类物质可对其进行调节。本研究的目的是验证马黛茶巴拉圭冬青（Ilex paraguariensis）是一种富含酚类化合物的植物物种，其调节体外巨噬细胞中以及健康受试者摄入绿色或烤巴拉圭茶输注液后获得的外周血单核细胞和单核细胞衍生的巨噬细胞中PON-2的基因表达和活性。将THP-1巨噬细胞与增加量的巴拉圭茶提取物或绿原酸和咖啡酸（1-10 μ mol/L）一起孵育。对20名健康妇女进行了马黛茶或水（对照）摄入的体内效应的急性（摄入后2 h）和短期（每天摄入7 d后）评价。结果：在一般情况下，两种马黛茶之间没有差异。1和3 μ mol/L的马黛茶提取物或绿原酸可增加THP-1巨噬细胞PON-2相关基因的表达（P < 0.05），而较高浓度（5和10 μ mol/L）仅增加活性。咖啡酸仅诱导PON-2活性。急性摄入马黛茶输液增加单核细胞中的相关基因表达和PON-2活性而饮用马黛茶7 d后，PON-2相关基因表达量增加（P < 0.05）（P < 0.05），并有增加单核细胞和单核细胞源性巨噬细胞PON-2活性的趋势。提示：绿色或烤巴拉圭茶可调节单核细胞和巨噬细胞PON-2酶的mRNA相对表达和活性，可能具有抗氧化应激作用。(C)2012 Elsevier Inc. All rights reserved.
Objective: Paraoxonase-2 (PON-2) is an intracellular antioxidant enzyme that can be modulated by polyphenols. The aim of this study was to verify whether yerba mate (Ilex paraguariensis), a plant species rich in phenolic compounds, modulates gene expression and the activity of PON-2 in macrophages in vitro and in monocytes from peripheral blood and monocyte-derived macrophages obtained after the ingestion of green or roasted yerba mate infusions by healthy subjects.Methods: THP-1 macrophages were incubated with increasing amounts of yerba mate extracts or chlorogenic and caffeic acids (1-10 mu mol/L). The in vivo effects of yerba mate or water (control) intakes were evaluated acutely (2 h after ingestion) and in the short term (after daily ingestion for 7 d) in 20 healthy women.Results: In general, there was no difference between the two kinds of yerba mate studied. Yerba mate extracts or chlorogenic acid at 1 and 3 mu mol/L increased PON-2 relative gene expression in THP-1 macrophages (P < 0.05), whereas higher concentrations (5 and 10 mu mol/L) increased the activity only. Caffeic acid induced PON-2 activity only. The acute ingestion of yerba mate infusions increased relative gene expression and PON-2 activity in monocytes (P < 0.05), whereas the consumption of yerba mate for 7 d increased PON-2 relative gene expression (P < 0.05) and had a tendency to increase PON-2 activity in monocytes and monocyte-derived macrophages.Conclusion: It is suggested that green or roasted yerba mate modulates positively the mRNA relative expression and activity of the PON-2 enzyme in monocytes and macrophages, which may prevent cellular oxidative stress. (C) 2012 Elsevier Inc. All rights reserved.