Glutarnate uptake in cultured astrocytes depends on age:: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H2O2
Glutarnate uptake in cultured astrocytes depends on age:: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H2O2
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DOI：
10.1016/s0047-6374(02)00069-6
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发表时间：
2002-07-01
影响因子：
5.3
通讯作者：
Souza, DO
中科院分区：
文献类型：
--
作者：
Gottfried, C;Tramontina, F;Souza, DO
相对较少的研究已经进行了调查谷氨酸和发展和/或衰老之间的关系。大鼠皮质星形胶质细胞培养物被用作研究发育过程中谷氨酸摄取的模型。体外培养10 ~ 40 d星形胶质细胞标志物胶质细胞酸性蛋白（GFAP）和S100 B的免疫含量增加，而S100 B的基础分泌减少，基础谷氨酸摄取随年龄增加而增加。暴露于过氧化氢降低谷氨酸摄取更有力地在40比10 DIV。此外，40 DIV星形胶质细胞表现出更早的损失的完整性（在6小时）比10 DIV星形胶质细胞（在24小时）H2 O2暴露后。此外，鸟苷刺激谷氨酸摄取仅在10 DIV星形胶质细胞。目前的工作表明，成熟的星形胶质细胞在文化提出了一些神经化学的变化，也观察到在老年动物的星形胶质细胞。这些结果有助于了解大脑老化过程中兴奋性毒性和氧化应激的一些后果。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Relatively few studies have been conducted to investigate the relationship between glutamate and development and/or aging. Rat cortical astrocyte cultures were used as a model to investigate glutamate uptake during development. The immunocontent of the markers glial fibrillary acidic protein (GFAP) and S100B increased, while basal secretion of S100B decreased, in astrocytes from 10 to 40 days in vitro (DIV), Basal glutamate uptake increased with age. Exposure to hydrogen peroxide decreased glutamate uptake more potently at 40 than 10 DIV. Moreover, 40 DIV astrocytes showed earlier loss of integrity (at 6 h) than 10 DIV astrocytes (at 24 h) after H2O2 exposure. Addition of guanosine stimulated glutamate uptake only in 10 DIV astrocytes. The present work shows that mature astrocytes in culture present some neurochemical alterations also observed in astrocytes of aged animals. These results can contribute to the understanding of some consequences of the excitotoxicity and oxidative stress during brain aging. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.