Regulation of the expression of the Epstein-Barr virus early gene BFRF1
Regulation of the expression of the Epstein-Barr virus early gene BFRF1
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DOI：
10.1016/j.virol.2005.11.046
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发表时间：
2006-03-30
期刊：
影响因子：
3.7
通讯作者：
Angeloni, A
中科院分区：
文献类型：
--
作者：
Granato, M;Farina, A;Angeloni, A
Epstein-Barr病毒（EBV）从潜伏期到裂解期的转换是由两种病毒反激活因子ZEBRA和RTA的表达协调的。BFRF1基因已被证明在eb病毒裂解周期的早期阶段进行转录。在这里，我们对BFRF1启动子进行了表征，表明转染斑马可以刺激BFRF1的表达，而RTA只有在转染质粒量远远超过足以刺激其他RTA应答基因表达的质粒量后才能诱导BFRF1。然而，在BFRF1的表达中，斑马和RTA之间的合作效应是显而易见的，因为RTA的转染可以挽救斑马蛋白突变体Z（S186A）的反激活能力，该突变体具有影响DNA结合区的替代。此外，我们在BFRF1启动子区域鉴定了一个斑马响应元件（ZRE）和一个rta响应元件（RRE）。(C) 2005爱思唯尔公司版权所有。
The switch from latency to lytic phase of Epstein-Barr virus (EBV) is coordinated by the expression of two viral transactivators known as ZEBRA and RTA. The BFRF1 gene has been shown to be transcribed during the early phases of EBV lytic cycle. Here, we characterized the BFRF1 promoter showing that ZEBRA transfection stimulated BFRF1 expression, whereas RTA induced BFRF1 only after the transfection of an amount of plasmid largely in excess than that sufficient to stimulate the expression of other RTA-responsive genes. However, a co-operative effect between ZEBRA and RTA in the expression of BFRF1 is evident since the transfection of RTA can rescue the transactivating capacity of a mutant of the ZEBRA protein, known as Z(S186A), that has a substitution affecting the DNA binding region. Moreover, we identified one ZEBRA-responsive element (ZRE) and one RTA-responsive element (RRE) within the BFRF1 promoter region. (C) 2005 Elsevier Inc. All rights reserved.