Extensive lymphopenia due to apoptosis of uninfected lymphocytes in acute measles patients
Extensive lymphopenia due to apoptosis of uninfected lymphocytes in acute measles patients
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DOI：
10.1007/s007050050683
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发表时间：
2000-01-01
影响因子：
2.7
通讯作者：
Tashiro, M
中科院分区：
文献类型：
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作者：
Okada, H;Kobune, F;Tashiro, M
麻疹感染病毒会诱导瞬时免疫抑制，这有时会导致致命的机会性感染。为了获得有关该机制的基本信息，我们检查了从婴儿到35岁的急性麻疹患者的外周血单核细胞（PBMC），在皮疹发作后的不同时间获得了淋巴细胞亚群数量的不同时间。通过流程仪。分析了数据中患者衰老与免疫抑制的严重程度之间的关系。在经典的麻疹病例中，在皮疹发作后很快消失了被感染的淋巴细胞，在孵化期内被发现为较小的化学，而在被感染的情况下皮疹后细胞持续更长的时间。在皮疹开始时，在所有麻疹病例中都出现了明显的淋巴细胞减少症，CD4（+）T细胞，CD8+T细胞，B细胞，中性粒细胞和单核细胞的细胞数量减少。 TN对比度，天然杀手（NK）细胞的数量增加并激活，这可能是淋巴细胞减少症的反应补偿性。与凋亡相关的分子（例如CD95（FAS）和与TNF相关的凋亡诱导配体受体受体（TRAIL-R））高度表达在最幸运的未感染的淋巴细胞的细胞表面上，并且还观察到DNA碎片在DNA片段中也观察到Into In In In In In In Intro into Into Intro孵育。 。这些结果表明，严重的淋巴细胞减少主要是由于凋亡引起的未感染血细胞的死亡。腔化的严重程度和持续时间是年龄依赖性的。幼儿的严重程度较小，而一岁以下的婴儿以及青少年和成年人的严重程度较差。从这些结果中可以提出，由于未感染细胞的细胞凋亡引起的显着淋巴细胞减少是由麻疹病毒感染引起的免疫抑制的主要原因之一，并且与疾病的年龄依赖性严重程度相关。
Infection with measles virus induces a transient immunosuppression, which occasionally results in fatal opportunistic infections. To obtain fundamental information about the mechanism, we examined peripheral blood mononuclear cells (PBMC) from acute measles patients aged from infants to 35 years old, obtained at various times from incubation periods to 103 days after onset of rash, for the number of lymphocyte subsets by flowcytometry. The data were analyzed for relationships between aging of the patients and the severity of immunosuppression.In classical measles cases, infected lymphocytes detected as a minor pupulation during the incubation period disappeared soon after onset of rash, whereas in the cases of serious illness, the infected cells persisted longer after the rash. At the onset of rash, remarkable lymphopenia had already occurred in all measles cases with reduction in cell numbers of CD4(+)T cells, CD8+T cells, B cells, neutrophils, and monocytes. Tn contrast, natural killer (NK) cells were increased in number and activated, which might be a response compensatory for the lymphopenia. Apoptosis-associated molecules such as CD95(Fas) and TNF-related apoptosis-inducing ligand-receptor (TRAIL-R) were highly expressed on the cell surface of most surviving non-infected lymphocytes, and DNA fragmentation was also observed upon incubation in vitro. These results suggested that the profound lymphopenia was primarily due to extended death of non-infected blood cells caused by apoptosis. The severity and duration of the lumphopenia were age-dependent; less severe in young children whereas much severer in infants under one year of age as well as adolescents and adults. From these results, it was suggested that remarkable lymphopenia due to apoptosis of uninfected cells is one of the principal causes for immunosuppression induced by measles virus infection, and is correlated with the age-dependent severity of the disease.