Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
Fabrication of mucoadhesive chitosan coated polyvinylpyrrolidone/cyclodextrin/clotrimazole sandwich patches for oral candidiasis.
复制标题
DOI：
10.1016/j.carbpol.2015.06.032
复制
发表时间：
2015-11
影响因子：
11.2
通讯作者：
Prasopchai Tonglairoum Patrojanasophon;T. Ngawhirunpat;T. Rojanarata;S. Panomsuk;R. Kaomongkolgit;P. Opanasopit
中科院分区：
文献类型：
--
作者：
Prasopchai Tonglairoum Patrojanasophon;T. Ngawhirunpat;T. Rojanarata;S. Panomsuk;R. Kaomongkolgit;P. Opanasopit
本研究旨在利用静电纺丝技术制备克霉唑（CZ）复合三明治纳米纤维。将壳聚糖-半胱氨酸（CS-SH）/聚乙烯醇（PVA）包覆在载CZ的聚乙烯吡咯烷酮（PVP）/羟丙基-β-环糊精（HPβCD）纳米纤维上，以提高纤维的粘附性能，实现药物的缓释。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和X射线衍射（XRD）对纳米纤维进行了表征。纳米纤维的机械和粘膜粘附性能，药物释放，抗真菌活性和细胞毒性也进行了评估。纤维为纳米级，具有良好的粘膜粘附性能。的XRPD揭示了无定形CZ的纳米纤维中的分子分散体。实现了CZ从纳米纤维的初始快速释放。此外，与较短的涂覆时间相比，涂覆较长时间的夹层纳米纤维导致较慢的释放速率。在5、15和30分钟时，载有CZ的纳米纤维比商业CZ锭剂显著更快地杀死果蝇，并且对于2小时的孵育是安全的。因此，这些纳米纤维可能是治疗口腔念珠菌病的有前途的候选者。
This study aims to fabricate clotrimazole (CZ)-composite sandwich nanofibers using electrospinning. The CZ-loaded polyvinylpyrrolidone (PVP)/hydroxypropyl-β-cyclodextrin (HPβCD) fiber was coated with chitosan-cysteine (CS-SH)/polyvinyl alcohol (PVA) to increase the mucoadhesive properties and to achieve a sustained release of the drug from the nanofibers. The nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffractometry (XRD). The nanofibers mechanical and mucoadhesive properties, drug release, antifungal activity and cytotoxicity were also assessed. The fibers were in the nanoscale with good mucoadhesive properties. The XRPD revealed a molecular dispersion of amorphous CZ in the nanofibers. The initial fast release of CZ from the nanofibers was achieved. Moreover, the sandwich nanofibers coated for longer times resulted in slower release rates compared with the shorter coating times. The CZ-loaded nanofibers killed theCandidasignificantly faster than the commercial CZ lozenges at 5, 15 and 30 min and were safe for a 2-h incubation. Therefore, these nanofibers may be promising candidates for the treatment of oral candidiasis.