Identification of possible genetic alterations in the breast cancer cell line MCF-7 using high-density SNP genotyping microarray.
Identification of possible genetic alterations in the breast cancer cell line MCF-7 using high-density SNP genotyping microarray.
复制标题
DOI：
10.4103/1477-3163.50886
复制
发表时间：
2009
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Wang HY;Greenawalt D;Cui X;Tereshchenko IV;Luo M;Yang Q;Azaro MA;Hu G;Chu Y;Li JY;Shen L;Lin Y;Zhang L;Li H
癌细胞系广泛用于各种研究。了解这些品系的遗传改变对于理解其生物学基础机制非常重要。然而，由于配对的正常组织通常无法进行比较，因此很难精确确定癌细胞系中的遗传改变。为了解决这个问题，开发了一种高效且可靠的方法。目的：建立高效可靠的细胞系遗传图谱实验系统。使用新开发的高通量多重基因分型方法，对广泛使用的乳腺癌细胞系 MCF-7 进行了基因分析，其中包含跨越 11 条完整染色体和其他两个小区域的 4,396 个单核苷酸多态性 (SNP)。 MCF-7 中纯合 SNP 的比例 (13.3%) 显着低于对照细胞系和 24 名正常人个体中的纯合 SNP 比例（分别为 25.1% 和 27.4%）。 MCF-7 中的纯合 SNP 成簇被发现。这些簇的大小明显大于基于随机等位基因组合的预期。在 MCF-7 的染色体 1p、1q、2q、6q、13、15q、16q、17q 和 18p 上发现了 14 个这样的区域，在小区域中发现了 2 个。这些结果通常与使用不同方法获得的结果一致，但可以更好地确定其染色体位置。所使用的方法提供了一种可靠的方法来检测没有配对正常组织的癌细胞系中可能的遗传改变。
Cancer cell lines are used extensively in various research. Knowledge of genetic alterations in these lines is important for understanding mechanisms underlying their biology. However, since paired normal tissues are usually unavailable for comparison, precisely determining genetic alterations in cancer cell lines is difficult. To address this issue, a highly efficient and reliable method is developed. Aims: Establishing a highly efficient and reliable experimental system for genetic profiling of cell lines. A widely used breast cancer cell line, MCF-7, was genetically profiled with 4,396 single nucleotide polymorphisms (SNPs) spanning 11 whole chromosomes and two other small regions using a newly developed high-throughput multiplex genotyping approach. The fractions of homozygous SNPs in MCF-7 (13.3%) were significantly lower than those in the control cell line and in 24 normal human individuals (25.1% and 27.4%, respectively). Homozygous SNPs in MCF-7 were found in clusters. The sizes of these clusters were significantly larger than the expected based on random allelic combination. Fourteen such regions were found on chromosomes 1p, 1q, 2q, 6q, 13, 15q, 16q, 17q and 18p in MCF-7 and two in the small regions. These results are generally concordant with those obtained using different approaches but are better in defining their chromosomal positions. The used approach provides a reliable way to detecting possible genetic alterations in cancer cell lines without paired normal tissues.